Mitogenicity of thrombin and surface alterations on mouse splenocytes

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Abstract

Splenocyte cultures prepared from BALB/c mice can be stimulated to DNA synthesis by thrombin and trypsin but not by bovine serum. The stimulation by thrombin as a function of concentration of the enzyme is the same in the presence or absence of serum, whereas lower concentrations of trypsin are not mitogenic if combined with serum. These observations indicate that the inactivating or neutralizing proteins for thrombin have been substantially reduced during the clotting process but that inhibitors of trypsin still remain. The stimulation of splenocytes to DNA synthesis is accompanied by a loss of a lactoperoxidase-iodinatable protein on the splenocyte cell surface. This protein is 45 000 D and corresponds in size to a group of lymphocyte surface proteins including H-2 antigen. We have proposed that thrombin may play a role in wound healing by stimulating proliferation of not only fibroblasts but also B lymphocytes, a process of possible physiological significance in defending the host against pathogens and during inflammations.

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This work was supported by grant CA02015 of the NCI, NIH to John M. Buchanan.

2

Lan Bo Chen is a predoctoral fellow of Johnson & Johnson Associate Industries Fund.

1

Nelson N. H. Teng is a postdoctoral fellow of the Muscular Dystrophy Association.

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