Elsevier

Life Sciences

Volume 22, Issue 20, 22 May 1978, Pages 1843-1852
Life Sciences

Opiate binding to membrane preparations of neuroblastoma X glioma hybrid cells NG108-15: Effects of ions and nucleotides

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Abstract

Interaction of a number of opiate agonists with the opiate receptors in NG108-15 cell membranes is influenced by ions, as well as certain nucleotides. Steady state binding of [3H]leu-enkephalin is increased by Mg++ and decreased by Na+, GMP-P(NH)P, GTP, GDP, ITP and IMP-P(NH)P. Half-maximal inhibition produced by GMP-P(NH)P occurred at 4.6 μM. The dissociation of [3H]leu- and [3H]met-enkephalin, as well as [3H]etorphine, from these opiate receptors was also shown to be altered by both ions and nucleotides.

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