Elsevier

Life Sciences

Volume 45, Issue 7, 1989, Pages 577-583
Life Sciences

Increased vascular contractile sensitivity to serotonin in spontaneously hypertensive is linked with increased turnover of phosphoinositide

https://doi.org/10.1016/0024-3205(89)90042-8Get rights and content

Abstract

This study was conducted to determine if increased vascular contractile sensitivity to serotonin in spontaneously hypertensive (SHR) rats is linked with increased phosphoinositide turnover. Aortic and mesenteric artery rings from SHR exhibited 6.2- and 5.0-fold greater contractile sensitivity to serotonin than the aortic and mesenteric artery rings from normotensive Wistar-Kyoto (WKY) rats. Serotonin-induced turnover of phosphoinositide was measured by quantifying the accumulation of [3H] inositol labeled monophosphate (IP), inositol bisphosphate (IP2) and inositol trisphosphate (IP3). Serotonin (3, 30, 200 μM) induced siginificantly greater accumulation of IP in SHR (279%, 590%, 895%) than in WKY (24%, 127%, 328%) aortic rings. Similarly, 3, 30 and 200 μM serotonin induced significantly greater accumulation of IP2 (118%, 241%, 451%) and IP3 (90%, 100%, 247%) in SHR than the accumualation of IP2 (15%, 58%, 122%) and IP3 (19%, 27%, 73%) in WKY aortic rings. Based on these data it is suggested that the greater vascular sensitivity to serotonin in SHR, at least in part, is attributable to increased turnover of phosphoinositide.

References (24)

  • Huzoor-Akbar et al.

    Thromb. Res.

    (1988)
  • Huzoor-Akbar et al.

    Thromb. Res.

    (1986)
  • Huzoor-Akbar et al.

    Biochem. Pharmacol.

    (1985)
  • M. Castagna et al.

    J. Biol. Chem.

    (1982)
  • M.J. Mackay et al.

    Biochem. Biophys. Res. Comm.

    (1987)
  • D.D. McGregor et al.

    Am. J. Physiol.

    (1970)
  • T.E. Mecca et al.

    Hypertension

    (1984)
  • B.L. Roth et al.

    Neuropharmacol.

    (1984)
  • T. Nakaki et al.

    J. Pharmacol. Exp. Ther.

    (1985)
  • B.L. Roth et al.

    J. Pharmacol. Exp. Ther.

    (1986)
  • R.J. Haslam et al.

    J. Receptor Res.

    (1984)
  • J.R. Williamson

    Hypertension

    (1986)
  • Cited by (25)

    • Signal transduction mechanisms of the vasoconstriction in hypertension

      1994, European Journal of Pharmacology: Molecular Pharmacology
    View all citing articles on Scopus
    View full text