Anticoagulant properties of heparin fractionated by affinity chromatography on matrix-bound antithrombin III and by gel filtration

doi:10.1016/0049-3848(76)90105-5

Thrombosis Research

Volume 9, Issue 6, December 1976, Pages 575-583

https://doi.org/10.1016/0049-3848(76)90105-5 Get rights and content

Abstract

Heparin purified by affinity chromatography on anti-thrombin III-Sepharose has been studied by various methods. The specific activities of the materials obtained were in the range 170–230 units/mg as determined by a whole plasma clotting method and 360–780 units/mg as determined by a F.X_a inhibition method. Gel filtration of the material showed that there was a definite molecular size dependency of the specific activities and the activity profiles were markedly different when assay -ed by different methods. These features were also observed (at generally lower activities) with gel filtration fractions of commercial heparin. The possible conclusions regarding the mechanism of heparin anticoagulant action are discussed.

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Anticoagulant properties of heparin fractionated by affinity chromatography on matrix-bound antithrombin III and by gel filtration

Abstract

Thromb. Res.

Thromb. Res.

Biochem. Biophys. Res. Comm.

Febs Letters

Actions and Interactions of Antithrombin and Heparin

The New England J. of Med.

On the proteinase inhibitors of human plasma with special reference to antithrombin