Journal of Allergy and Clinical Immunology
Immunochemical analysis of sulfonamide drug allergy: Identification of sulfamethoxazole-substituted human serum proteins☆,☆☆,★,★★
Section snippets
Rabbit immunizations/antibody purification
Hunter's Titermax adjuvant was obtained from CytRx Corporation (Norcross, Ga.). SMX-bovine serum albumin (BSA) (Sigma Chemical Co., St. Louis, Mo.) was prepared according to the method described previously4 and was used as the immunizing antigen. An Econo-Pak Protein A kit from Bio-Rad (Richmond, Calif.) was used for the purification of the rabbit IgG.
Enhanced ELISA
Borate-buffered saline, phosphate buffered saline (PBS), blocking solution/standard diluent (ovalbumin, 5 gm/L in PBS/Tween), PBS/Tween (Tween
Characterization of rabbit anti-SMX antibodies
SMX-specific polyclonal antibodies were generated in New Zealand white rabbits. After animals were immunized and boosted with SMX-BSA (prepared by covalent linkage of the diazonium salt of SMX to BSA), serum was obtained, partially purified, and used in inhibition ELISAs. Prebleed serum was also obtained and partially purified for use as a negative control. Before addition to microtiter plates containing antigen, antibodies diluted 1:24,000 were incubated with one of three inhibitors: BSA,
DISCUSSION
A significant number of adverse drug reactions appear to be immune-mediated, and as for any immune-mediated event involving simple haptens, induction and elicitation of the reaction requires the formation of immunogenic conjugates. Although it has been assumed that these conjugates are formed through covalent binding of the drug hapten itself or a reactive intermediate to tissue macromolecules, knowledge of the metabolic processes involved in the generation of these immunogenic conjugates is
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Cited by (0)
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From aUniversity of Texas Southwestern Medical Center, Dallas; and bEmory University School of Medicine, Atlanta.
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Supported by grant AI33711-02 from the National Institutes of Health.
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Reprint requests: Rebecca S. Gruchalla, MD, PhD, Department of Internal Medicine, Division of Allergy, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-8859.
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