Elsevier

Neuropeptides

Volume 1, Issue 1, July 1980, Pages 53-62
Neuropeptides

Demonstration of a putative receptor site for cholecystokinin in rat brain

https://doi.org/10.1016/0143-4179(80)90009-8Get rights and content

Abstract

High affinity (apparent Kd ⋍ 0.65 nM) and saturable (Bmax ⋍ 6.6 fmoles/mg protein) binding sites for cholecystokinin (CCK) have been demonstrated in crude synaptosomal membranes from rat cerebral cortex. Scatchard analysis of the equilibrium binding data indicates a single population of non-interacting sites. The specific binding of 125I-CCK33 to brain membranes is reversible (t12 ⋍ 6 minutes), and can be inhibited by structurally related CCK analogues but not by unrelated neuropeptides or drugs. Specific CCK binding reaches equilibrium at a temperature-dependent rate, and is abolished when membranes are pre-treated with heat. Kinetic and competition studies suggest that these high affinity binding sites represent physiologically-relevant receptors for CCK in brain.

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