Morphine effect on proliferation of normal and tumor cells of immune origin

doi:10.1016/0165-2478(93)90055-7

Immunology Letters

Volume 36, Issue 2, May 1993, Pages 215-218

https://doi.org/10.1016/0165-2478(93)90055-7 Get rights and content

Abstract

The influence of morphine on proliferation of human tumor K562 and lymphoid cells was studied and compared with that on the mitogen-induced proliferation of human peripheral blood mononuclear cells (PBMC). Morphine was shown to act as a suppressor of both cellular DNA synthesis (50% and more as compared to control) and the cellular population growth of mitogen-induced PBMC, B-lymphoma Namalva cells and EBV-transformed lymphocytes. Morphine activated proliferation of myeloid K562 and T-lymphoma Yurkat cells 1.5-fold. It is supposed that the opposite effects of morphine on proliferation of cell lines of immune origin reveal the difference in modulation of diverse immune cell types by morphine.

References (12)

J.E. Morley et al.
Life Sci.
(1987)
H. Ovadia et al.
J. Neuroimmunol.
(1989)
E. Fiorica et al.
Life Sci.
(1988)
P. Borboni et al.
BBRC
(1989)
I.S. Zagon et al.
Life Sci.
(1988)
I. Zagon et al.
Science
(1983)

There are more references available in the full text version of this article.

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Morphine effect on proliferation of normal and tumor cells of immune origin

Abstract

Life Sci.

J. Neuroimmunol.

Life Sci.

BBRC

Life Sci.

Science