The detection of β-adrenoceptors on murine lymphocytes

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Abstract

The β-adrenoceptor antagonist (125I)-iodohydroxybenzyl pindolol was used to detect and characterise β-adrenoceptors on the surface of mouse lymphocytes by a direct radioligand receptor assay using intact viable cells. The binding was stereoselective and appeared to be with a β2-type receptor as shown by competition with a number of adrenergic reagents. Scatchard analysis of the ligand binding demonstrated approximately 3000 binding sites per cell with an average dissociation constant (Kd) of 0.92 nM. Little variation in binding characteristics was observed between lymphocytes from individual mice of either sex or in 24 different inbred mouse strains. In addition no significant differences were noted between T and B enriched lymphocyte populations, i.e. there was no functional polymorphism of the β-adrenoceptor on mouse lymphocytes. Thymocytes carried a lower affinity receptor which may be related to their functional immaturity. By using antisera specific for murine cell surface alloantigens, the β-adrenoceptor was shown not to be associated with the H-2K, H-2D, Ia, Ly−1, −2, −3, −4, or −5, Tla or Thy antigens. However, specific antisera for the Ly-7 antigen did partially inhibit IHYP binding. The results show that lymphocytes have a β2-adrenoceptor which closely resembles the receptor on other tissues.

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