Expression of the LH/CG receptor gene in rat ovarian tissue is regulated by an extensive alternative splicing of the primary transcript
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Cited by (67)
LHR splicing variants and gene expression in the marmoset monkey
2007, Molecular and Cellular EndocrinologyThe tale of follitropin receptor diversity: A recipe for fine tuning gonadal responses?
2007, Molecular and Cellular EndocrinologyExpression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages
2005, PlacentaCitation Excerpt :Immunohistochemistry was performed using the streptavidin–biotin method with an LSAB kit (Dako). Rabbit polyclonal antibody raised against a synthetic peptide (15–38) encoding exon 1 of the rat luteal LH/CG receptor was kindly provided by Dr. C.V. Rao (University of Louisville Health Science Center, Louisville, KY, USA) [10,15]. The rat LH/CG receptor has 85% homology with the human LH/CG receptor [12].
An alternative use of basic pGEX vectors for producing both N- and C-terminal fusion proteins for production and affinity purification of antibodies
2005, Protein Expression and PurificationIdentification and Structural Characterization of the Neuronal Luteinizing Hormone Receptor Associated with Sensory Systems
2004, Journal of Biological ChemistryCitation Excerpt :Three major products of 1.7, 1.8, and 1.9 kb were obtained from the adult testis (lane 1), ovary (lane 11), and placenta (lane 10), whereas muscle (lane 13) and liver (lane 14) showed no amplification of the LHR mRNA. The 1.9- and 1.7-kb species correspond to the full-length LHR and the most common receptor isoform, respectively (24–26), whereas the 1.8-kb species most likely represents a hybrid of the 1.9- and 1.7-kb products (27). Because of the nested primers used in the second amplification run, the final products were slightly (∼200 bp) smaller than the original cDNA.
Decreased luteinizing hormone receptor mRNA expression in human ovarian epithelial cancer
2000, Gynecologic Oncology