Elsevier

Neuroscience

Volume 35, Issue 3, 1990, Pages 577-591
Neuroscience

The distribution and cellular localization of the serotonin 1C receptor mRNA in the rodent brain examined by in situ hybridization histochemistry. Comparison with receptor binding distribution

https://doi.org/10.1016/0306-4522(90)90330-7Get rights and content

Abstract

The regional distribution and cellular localization of mRNA coding for the serotonin 1C receptor were investigated in tissue sections of mouse and rat brain by in situ hybridization histochemistry. Several32P-labelled riboprobes derived from mouse genomic clones were used. The serotonin 1C receptor binding sites were visualized autoradiographically and quantified using [3H]mesulergine as ligand, in the presence of spiperone to block serotonin 1C receptors. Strong hybridization signal was observed in the choroid plexus of all brain ventricles. High levels of hybridization were also seen in the anterior olfactory nucleus, pyriform cortex, amygdala, some thalamic nuclei, especially the lateral habenula, the CA3 area of the hippocampal formation, the cingulate cortex, some components of the basal ganglia and associated areas, particularly the nucleus subthalamicus and the substantia nigra. The midbrain and brainstem showed moderate levels of hybridization. The distribution of the serotonin 1C receptor mRNA corresponded well to that of the serotonin 1C receptors. The highest levels of the serotonin 1C receptor binding were observed in the choroid plexus. In addition, significant levels of the serotonin 1C receptor binding were seen in the anterior olfactory nucleus, pyriform cortex, nucleus accumbens, ventral aspects of the striatum, paratenial and paracentral thalamic nuclei, amygdaloid body and substantia nigra pars reticulata. The cingulate and retrosplenial cortices as well as the caudal aspects of the hippocampus (CA3) were also labelled. Binding in brainstem and medulla was low and homogeneously distributed. No significant binding was seen in the habenular and subthalamic nuclei. Similar findings were obtained in rat brain.

These results demonstrate that, in addition to their enrichment in the choroid plexus, the serotonin 1C receptor mRNA and binding sites are heterogeneously distributed in the rodent brain and thus could be involved in the regulation of many different brain functions. The combination of in situ hybridization histochemistry with receptor autoradiography opens the possibility of examining the regulation of the serotonin 1C receptor synthesis after pharmacological or physiological alterations.

Reference (53)

  • MotohashiN. et al.

    Functional mapping of the effects of lesions of the habenular nuclei and their afferents in the rat

    Brain Res.

    (1986)
  • PazosA. et al.

    Mesulergine, a selective serotonin-2 ligand in the rat cortex, does not label these receptors in porcine and human cortex: evidences for species differences on brain serotonin-2 receptors

    Eur. J. Pharmac.

    (1984)
  • PazosA. et al.

    The binding of serotonergic ligand to the porcine choroid plexus: characterization of a new type of serotonin recognition site

    Eur. J. Pharmac.

    (1984)
  • PazosA. et al.

    Serotonin receptors in the human brain. III Autoradiographic mapping of serotonin-1 receptors

    Neuroscience

    (1987)
  • PazosA. et al.

    Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors

    Brain Res.

    (1985)
  • PazosA. et al.

    Quantitative autoradiographic mapping of serotonin receptors in the rat brain. II. Serotonin-2 receptors

    Brain Res.

    (1985)
  • PeroutkaS.J.

    5-Hydroxytryptamine receptor subtypes: molecular, biochemical and physiological characterization

    Trends Neurosci.

    (1988)
  • QuattroneA. et al.

    Pharmacological evidence of supersensitivity of central serotoninergic receptors involved in the control of prolactin secretion

    Eur. J. Pharmac.

    (1981)
  • SoubriéP. et al.

    Role of the lateral habenula in modulating nigral and striatal in vivo [3H]serotonin-release in the cat

    Brain Res.

    (1981)
  • SpecialeS.G. et al.

    Habenular modulation of raphe indoleamine metabolism

    Life Sci.

    (1980)
  • SternW.C. et al.

    Neuropharmacology of the afferent projections from the lateral habenula and substantia nigra to the anterior raphe in the rat

    Neuropharmacology

    (1981)
  • Van der KooyD. et al.

    The organization of the efferent projections and striatal afferents of the entopenduncular nucleus and adjacent areas in the rat

    Brain Res.

    (1981)
  • BrewertonT.D. et al.

    Induction of migrainelike headaches by the serotonin agonist m-chlorophenylpiperazine

    Clin. Pharmac. Ther.

    (1988)
  • ConnP.J. et al.

    Agonist-induced phosphoinositide hydrolysis in choroid plexus

    J. Neurochem.

    (1986)
  • DerkachV. et al.

    5-HT3 receptors are membrane ion channels

    Nature

    (1989)
  • DohlmanH.G. et al.

    A family of receptors coupled to guanine nucleotide regulatory proteins

    Biochemistry

    (1987)
  • Cited by (217)

    • Behavioral characteristics of 5-HT<inf>2C</inf> receptor knockout mice: Locomotor activity, anxiety-, and fear memory-related behaviors

      2020, Behavioural Brain Research
      Citation Excerpt :

      Seven families of serotonin 5-HT receptors comprising a total of 14 subtypes have been identified, and each subtype has distinct functions [1]. Of these subtypes, the serotonin 5-HT2C receptor has attracted considerable attention in brain research because the 5-HT2C receptor is highly expressed in several brain regions involved in emotional functions [2]. To examine the roles of the 5-HT2C receptor in emotional functions, several pharmacological tools have been developed [3–5].

    View all citing articles on Scopus
    View full text