Molecular cloning of rat trkC and distribution of cells expressing messenger RNAs for members of the trk family in the rat central nervous system

doi:10.1016/0306-4522(92)90292-A

Neuroscience

Volume 51, Issue 3, December 1992, Pages 513-532

https://doi.org/10.1016/0306-4522(92)90292-A Get rights and content

Abstract

Tyrosine protein kinases trk, trkB and trkC are signal-transducing receptors for the neurotrophins nerve growth factor, brain-derived nerve growth factor, neurotrophin-3 and neurotrophin-4. Here we report on the isolation of cDNA fragments encoding a part of rat trk and irkB proteins, respectively, and characterization of a full-length cDNA clone encoding rat trkC. Cells expressing mRNAs for the different members of the trk family were identified in the rat central nervous system by in situ hybridization using oligonucleotide probes designed from the isolated cDNA sequences and complementary to mRNA sequences coding for the extracellular region of the receptors. The expression of trk mRNA was found to be restricted to neurons of the basal forebrain, caudate-putamen with features of cholinergic cells and to magnocellular neurons of several brainstem nuclei. In contrast, cells expressing trkB and trkC mRNAs were widely distributed in the brain. Areas expressing high levels of trkB or trkC mRNAs included olfactory formations, neocortex, hippocampus, thalamic and hypothalamic nuclei, brainstem nuclei, cerebellum and spinal cord motoneurons. A similar distribution for trkB and trkC mRNAs was shown in most areas but each probe specific for these mRNAs also provided distinct labeling patterns in different subregions, layers and cells.

Comparison between our data and previous analyses of cells expressing mRNAs for neurotrophins and the low-affinity nerve growth factor receptor suggests that different modes of action and different combinations of receptors mediate biological responses to neurotrophins in the adult rat brain.

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^*: Present address: URA-CNRS1200, Laboratoire d'Histologie-Embryologie, Universitéde Bordeaux II, 33076 Bordeaux, France.

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Molecular cloning of rat trkC and distribution of cells expressing messenger RNAs for members of the trk family in the rat central nervous system

Abstract

Neuron

Neuron

Neuron

Devl Brain Res.

Cell

Neurosci. Lett.

Brain Res.

Neuron

Neuroscience

Devl Biol.

Expl Neurol.

Neuron

Neuron

Neuron

Brain Res.

Neuron

Cell

Neuron

Cell

Brain Res.

Cell

Fedn Eur. Biochem. Socs. Lett.

Neuroscience

Cell

Cell

Neuron

Cell

J. molec. Biol.

Cell

Expl Neurol.

Neuron

Neuron

Neuron

Neuroscience

Trends Neurosci.

Brain Res.

Neuroscience

Neuroscience

Neuroscience

Neuron

Brain Res.

Brain Res.

Brain Res.

Cell

Cell

J. biol. Chem.

Binding of nerve growth factor receptor in sympathetic ganglia

Purification of a new neurotrophic factor from mammalian brain

Eur. molec. biol. Org. J.

Nerve growth factor receptor and choline acetyltransferase colocalization within the rat forebrain: response to fimbria-fornix transection

J. comp. Neurol.

Expanded distribution of mRNA for nerve growth factor, brain-derived neurotrophic factor and neurotrophin-3 in the rat brain after colchicine treatment