Elsevier

Gene

Volume 144, Issue 2, 8 July 1994, Pages 237-241
Gene

Short communication
Insertion of the human cytomegalovirus enhancer into a myeloproliferative sarcoma virus long terminal repeat creates a high-expression retroviral vector

https://doi.org/10.1016/0378-1119(94)90383-2Get rights and content

Abstract

A new retroviral vector is characterized in which the enhancer in the long terminal repeat of myeloproliferative sarcoma virus (MPSV) is combined with the strong and universally active immediate-early human cytomegalovirus (hCMV) enhancer. We demonstrate that insertion of the hCMV enhancer increases the amount of vector-specific mRNA in various rodent cell lines and in human diploid fibroblasts, by at least 3–5-fold. The vector may be particularly useful if high expression of two genes is desired.

References (28)

  • C. Auffray et al.

    Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor DNA

    Eur. J. Biochem.

    (1980)
  • S.J. Baker et al.

    Suppression of human colorectal carcinoma cell growth by wild-type p53

    Science

    (1990)
  • L. Cheng et al.

    In vivo promoter activity and transgene expression in mammalian somatic tissues evaluated by using particle bombardment

  • J.E. DeLarco et al.

    Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation

    J. Cell. Physiol.

    (1978)
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