Elsevier

Gene

Volume 161, Issue 2, 19 August 1995, Pages 271-275
Gene

Short communication
Cloning of the cDNA encoding human C/EBPγ, a protein binding to the PRE-I enhancer element of the human interleukin-4 promoter

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Abstract

The positive regulatory element-I (PRE-I) is a strong enhancer element essential for expression of the human interleukin-4 (IL-4)-encoding gene. In order to identify transcription factors binding to PRE-I, we screened a cDNA expression library from human Jurkat T-cells. A cDNA encoding the human CCAAT/enhancer binding protein-γ (hC/EBPγ) was cloned. The deduced amino acid (aa) sequence of hC/EBPγ contains 150 aa with high homology to mouse Ig/EBP-1 and rat C/EBPγ. The mRNA of hC/EBPγ is expressed at a high level in Jurkat T-cells in three forms generated via differential polyadenylation. DNA-binding experiments with recombinant protein produced in bacteria demonstrate that hC/EBPγ binds to PRE-I, but not to unrelated DNA fragments. Our data also show that hC/EBPγ may cooperate with Fos to bind to PRE-I

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Permanent address: Shemyakin Institute of Bioorganic Chemistry, 142292 Puschino, Russia

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