Neuron
Electrophysiology of a chick neuronal nicotinic acetylcholine receptor expressed in xenopus oocytes after cDNA injection
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Cited by (111)
Analysis of neuronal nicotinic acetylcholine receptor α4β2 activation at the single-channel level
2016, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :In single channel recordings of transfected cells, we determined two conductances for channel openings, a low-conductance channel with a chord conductance of 29 pS and a high-conductance channel with 44 pS (Fig. 2). Our values are in the range already published using different species and techniques [18,41,43–45,50–55]. Most of them observed 2 conductances and even 3 different ones.
Conduits of life's spark: A perspective on ion channel research since the birth of neuron
2013, NeuronCitation Excerpt :In reflecting on the questions that motivated ion channel research 25 years ago, it is striking that the spirit, if not the details, of the studies exemplified in Neuron’s inaugural year mark many of the same questions that occupy the field today. These include: what is the physical nature of a channel (Auld et al., 1988; Ballivet et al., 1988; Deneris et al., 1988; Levitan et al., 1988; Lotan et al., 1988; Rudy et al., 1988; Timpe et al., 1988)? How do ions and pharmacological tools interact with channel pores (MacKinnon et al., 1988; Miller, 1988; Miller et al., 1988)?
Nicotinic acetylcholine receptors: From basic science to therapeutics
2013, Pharmacology and TherapeuticsA review of experimental techniques used for the heterologous expression of nicotinic acetylcholine receptors
2009, Biochemical PharmacologyCitation Excerpt :In later studies, functional expression was achieved in Xenopus oocytes using cRNA that had been transcribed in vitro from nAChR subunit cDNAs, using purified RNA polymerase [80]. In addition, a more direct approach of injecting cDNA directly into the oocyte nucleus has also been used successfully for the functional expression of nAChRs, first being used to characterize the neuronal α4β2 nAChR [81]. An approach that has been used extensively to increase expression levels of nAChRs (and of other recombinant proteins) in Xenopus oocytes is to replace the 5′ untranslated region (UTR) of the cDNA/cRNA with the corresponding UTR of a gene such as Xenopus β-globin [82].
Exocytic trafficking is required for nicotine-induced up-regulation of α<inf>4</inf>β<inf>2</inf> nicotinic acetylcholine receptors
2005, Journal of Biological ChemistryCitation Excerpt :At 24 h post-transfection, live cells were labeled with antibodies directed against the extracellular N-terminal domain of the α4 subunit to specifically label surface-expressed receptors. Since neither α4 nor β2 is capable of assembling into functional homo-oligomeric receptors (29), no surface labeling was observed when cells were mock-transfected or transfected with either α4 or β2 subunits alone (data not shown). However, when transfected together, mouse α4 and β2 subunits formed receptors that were detected at the surface of HEK293T cells by immunofluorescence with antibodies against the α4 (mAb 299) subunit (Fig. 1A).
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Permanent address: Department of Physiology, McGill University, Montreal, Canada.