Neuron
Volume 5, Issue 2, August 1990, Pages 121-126
Journal home page for Neuron

Article
21-Aminosteroids attenuate excitotoxic neuronal injury in cortical cell cultures

https://doi.org/10.1016/0896-6273(90)90302-VGet rights and content

Abstract

We studied the protective efficacy of novel 21-aminosteroids against several forms of neuronal injury in murine cortical cell cultures. Concentrations of 200 nM to 20 μM partially attenuated the damage induced by glucose deprivation, combined oxygen-glucose deprivation, or exposure to NMDA; maximal protection was less than that produced by NMDA antagonists, but the combination of a 21-aminosteroid plus an NMDA antagonist produced a greater benefit than either drug alone. 21-Aminosteroid addition did not attenuate NMDA-induced whole-cell current, but did block almost all of the damage induced by exposure to iron, a protective action consistent with inhibition of free radical-mediated lipid peroxidation. Lipid peroxidation may be a downstream event mediating a portion of the injury triggered by excess stimulation of NMDA receptors.

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    Present address: laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, INF 282, D-6900 Heidelberg, Federal Republic of Germany.

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