European Journal of Pharmacology: Molecular Pharmacology
Short communicationCloning and fnctional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit
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Cited by (150)
5-HT2C receptors in psychiatric disorders: A review
2016, Progress in Neuro-Psychopharmacology and Biological PsychiatryTherapeutics of 5-HT<inf>3</inf> receptor antagonists: Current uses and future directions
2011, Pharmacology and TherapeuticsCitation Excerpt :A large intracellular loop between TM3 and TM4 is present, but is not required for the expression of the homomeric A receptor in the plasma membrane or for its function (Jansen et al., 2008), based upon its substitution with a heptapeptide derived from the short intracellular loop between TM3 and TM4 in the prokaryotic homolog of the ion channel superfamily (Bocquet et al., 2007). The large intracellular loop of rodent A subunits is a site for alternative splicing (Hope et al., 1993; Isenberg et al., 1993; Lankiewicz et al., 1998). The large intracellular loops of the 5-HT3 receptor subunits also contain multiple consensus sites for protein kinase dependent phosphorylation (Maricq et al., 1991; Davies et al., 1999; Dubin et al., 1999; Karnovsky et al., 2003; Niesler et al., 2003).
Genetic Organization of the Serotonergic System
2010, Handbook of Behavioral NeuroscienceCitation Excerpt :Interestingly, the mouse 5-HT3A gene intron-exon organization demonstrated similarities to muscular and neuronal nicotinic acetylcholine receptor genes (Wada et al., 1988). Additionally, an alternative use of exon 9 splice acceptor sites results in the deletion of 6 amino acids in the large intracellular loop between transmembrane 3 and transmembrane 4, therefore generating a short (m5-HT3A(a) receptor) and a long (m5-HT3A(b) receptor) isoform of the murine m5-HT3A receptor (Hope et al., 1993; Uetz et al., 1994; Werner et al., 1994). The m5-HT3A(a) mRNA expression is about five times higher than the m5-HT3A(b) mRNA in both neuroblastoma and neuronal tissues (Werner et al., 1994).
The 5-HT<inf>3</inf> receptor - the relationship between structure and function
2009, NeuropharmacologyCitation Excerpt :The human HTR3A gene comprises 9 exons and 8 introns and is located upon chromosome 11 (Weiss et al., 1995) (Table 1). Alternative splicing of the primary RNA transcript encoding mouse, rat and guinea pig, but not human, dog, or ferret, 5-HT3A subunits results in ‘long’ (5-HT3A(a)) and short (5-HT3A(b)) isoforms (Hope et al., 1993; Isenberg et al., 1993; Werner et al., 1994; Belelli et al., 1995; Lankiewicz et al., 1998; Mochizuki et al., 2000; Jensen et al., 2006). The 5-HT3A(b) isoform lacks 6 (mouse and guinea pig), or 5 (rat) amino acid residues within the large intracellular loop linking M3 and M4.