European Journal of Pharmacology: Molecular Pharmacology
Pharmacological properties of two recombinant splice variants of the PACAP type I receptor, transfected and stably expressed in CHO cells
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C-terminal amidation of PACAP-38 and PACAP-27 is dispensable for biological activity at the PAC1 receptor
2016, PeptidesCitation Excerpt :PACAP, the endogenous agonist of the PAC1 receptor, exists in vivo as either a 27 or 38 residue peptide (PACAP-27 or -38), depending on extent of processing of its prohormone precursor (Fig. 1). To confirm that these two forms of PACAP are similar in both potency and intrinsic activity at the PAC1 receptor in a cell-based assay, as previously established in cell-free receptor binding assays [7], we examined PACAP-induced cyclic AMP elevation in neuroendocrine cell line NS-1 that natively expresses PAC1 receptors but do not express the highly homologous VPAC1 or VPAC2 receptors [15]. For real-time measurements of cyclic AMP production, NS-1 cells were transduced with a retroviral vector encoding a cAMP biosensor (CBS) that catalyzes the oxidization of luciferin to yield a luminescent signal that is proportional to cAMP levels.
Identification of PAC1 receptor isoform mRNAs by real-time PCR in rat suprachiasmatic nucleus
2002, Molecular Brain ResearchA combination assay for simultaneous assessment of multiple signaling pathways
1999, Journal of Pharmacological and Toxicological Methods