Biochemical and Biophysical Research Communications
There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini
Section snippets
Materials and methods
Identification of novel receptors. Sequences of known GPCRs from LN-7TM family (BAI1; NP_001693.1, BAI2; NP_001694.1, BAI3; NP_001695.1, CELSR1; NP_055061.1, CELSR2; NP_001399.1, CELSR3; NP_001398.1, CD97; NP_001775.1, EMR1; NP_001965.1, EMR2; NP_038475.1, EMR3; NP_115960.1, ETL; NP_071442.1, GPR97; AY140959, GPR110; AY140952, GPR111; AY140953, GPR112; AY140954, GPR113; AY140955, GPR114; AY140956, GPR115; AY140957, GPR116; AY140958, HE6 (GPR64); NP_005747.1, LEC1; NP_036434.1, LEC2; NP_055736.1
Results
We have recently published eight new LN-7TM receptors that were found through creation of Hidden Markov Models (HMM) based on previously known clan B and LN-TM7 receptors and a subsequent search of the human NCBI genome (http://www.ncbi.nlm.nih.gov). The Celera genome (http://www.celera.com) is however not searchable using HMMs. We therefore used the new sequences and the other LN-7TM sequences as baits for BLAST searches in the Celera genome. The result of the searches was six novel human
Discussion
The six novel receptors show clear overall structural and phylogenetic relationship to the LN-7TM receptors. Three of the new receptors (GPR123, GPR124, and GPR125) form a new separate cluster among the other LN-7TM receptors as shown in Fig. 1. Two of the receptors (GPR126 and GPR128) show phylogenetic relationship to HE6 and GPR56, which have been known for a few years, and three receptors that we recently described after searching the NCBI database with HMM models [10]. The sixth receptor
Acknowledgements
The studies were supported by the Swedish Research Council (VR, medicine), the Swedish Society for Medical Research (SSMF), Åke Wibergs Stiftelse, Petrus och Augusta Hedlunds Stiftelse, Svenska Läkaresällskapet, and Melacure Therapeutics AB, Uppsala, Sweden.
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