Adhesion promotes transcellular leukotriene biosynthesis during neutrophil-glomerular endothelial cell interactions: Inhibition by antibodies against CD18 and L-selectin

doi:10.1016/S0006-291X(05)81548-7

Biochemical and Biophysical Research Communications

Volume 186, Issue 3, 14 August 1992, Pages 1307-1314

https://doi.org/10.1016/S0006-291X(05)81548-7 Get rights and content

Summary

Eicosanoid formation by transcellular routes can amplify the levels and types of lipid mediators within a local milieu. To evaluate the role of adhesion in this process, we assessed the influence of mAb against adhesion molecules on LTC₄ generation by PMN-endothelial cell interaction. Transcellular LTC₄ generation was initiated by addition of fMLP to coincubations of GM-CSFprimed PMN and TNF-activated endothelial cells cultured from kidney glomeruli. Both PMN-endothelial cell adhesion and transcellular LTC₄ generation were inhibited by mAb against leukocyte L-selectin and CD18. These results indicate that cytokine-treated PMN and endothelial cells generate LTC₄ via transcellular routes by receptor-triggered mechanisms. They suggest that adhesion promotes transcellular eicosanoid biosynthesis and that adhesion molecules may also be targets for blockade of transcellular biosynthesis of lipid mediators.

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Adhesion promotes transcellular leukotriene biosynthesis during neutrophil-glomerular endothelial cell interactions: Inhibition by antibodies against CD18 and L-selectin

Summary

Science Wash. DC

Eur. J. Biochem.

J. Biol. Chem.

Adv. Prost. Thromb. Leuk. Res.

Eur. J. Biochem.

J. Exp. Med.

Nature

Physiol. Rev.