Original ArticlesSister of P-glycoprotein expression in different tissues
Section snippets
RT-PCR
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol. It was prepared from freshly excised porcine brain grey cortex, liver, kidney cortex, small- and large-gut mucosa. RNA from confluent (10 days in culture) porcine brain capillary endothelial cells was also examined. Primary cultures of these endothelial cells were prepared as described earlier [9]. After DNase digestion, RNA was quantified using a GeneQuant photometer (Pharmacia). Its integrity
Results and discussion
Brain grey cortex, liver, kidney, small- and large-gut mucosa are known to express high levels of Pgps 3, 10, 11, 12, 13, 14, 15, 16. The distribution of spgp was therefore investigated in these tissues. With RT-PCR, spgp expression could be demonstrated in brain, liver, small and large gut, whereas no spgp could be detected in the kidney (Fig. 1). The identity of the gene product with spgp was confirmed by cDNA sequence analysis. To determine which types of Pgps are expressed by these porcine
Acknowledgements
This work was supported by the Swiss National Science Foundation (Grant 32-052918.97), a scholarship to M.T. from the Association of Chemical Industries Basel, research grants from the Sandoz Foundation, and the ASTRA Research Funds of the Dept. of Internal Medicine of the University Hospital, Basel. We also thank U. Behrens for excellent technical assistance and I. Arnet for many valuable discussions.
References (20)
- et al.
Disruption of the mouse mdr1a P-glycoprotein gene leads to a deficiency in the blood–brain barrier and to increased sensitivity to drugs
Cell
(1994) - et al.
Duplication and evolution of the P-glycoprotein genes in pig
Biochim Biophys Acta
(1996) - et al.
The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver
J Biol Chem
(1998) - et al.
Multidrug resistance and the role of P-glycoprotein knockout mice
Eur J Cancer
(1995) - et al.
P-glycoprotein as the drug efflux pump in primary cultured bovine brain capillary endothelial cells
Life Sci
(1992) - et al.
Role of P-glycoprotein as a secretory mechanism in quinidine absorption from rat small intestine
J Pharm Sci
(1998) - et al.
Transport of bile acids in a human intestinal epithelial cell line, Caco-2
Biochim Biophys Acta
(1990) - et al.
Biochemistry of multidrug resistance mediated by the multidrug transporter
Annu Rev Biochem
(1993) - et al.
Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissue
Proc Natl Acad Sci USA
(1987) - et al.
Identification of members of the P-glycoprotein multigene family
Mol Cell Biol
(1989)