Glucagonlike peptide-2 enhances small intestinal absorptive function and mucosal mass in vivo

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Abstract

Purpose: Glucagonlike peptide-2 (GLP-2) is a 33-amino acid peptide that appears to be highly tissue specific for the intestine. This study was designed to examine the effect of systemically administered GLP-2 on intestinal absorptive function and mucosal mass, and determine the in vivo dose-response curves for this new peptide.

Methods: Twenty-five young adult male Sprague-Dawley rats had placement of jugular venous catheters connected to subcutaneously placed osmotic minipumps. The rats were divided into five groups based on the contents in the osmotic pump: group 1 (control, n = 5) normal saline and groups 2, 3, 4, and 5 (n = 5 each) were given GLP-2 at 5, 50, 250, and 500 μg/kg/d, respectively. After a 14-day infusion, [C14] galactose and [C14] glycine absorption were measured in a 10-cm segment of midsmall intestine using an in vivo closedrecirculation technique. Mucosal DNA content and protein content of the same small bowel segment were also determined for each group. Statistical analysis was performed by analysis of variance (ANOVA).

Results: GLP-2 significantly increased galactose absorption at a dose of 50 (P < .01), 250 (P < .01), and 500 (P < .05) μg/kg/d and glycine absorption at a dose of 50, 250, and 500 μg/kg/d (P < .01). GLP-2 also significantly increased mucosal DNA content at a dose of 50 (P < .01) and 250 (P < .05) μg/kg/d and protein content at a dose of 50 and 250 μg/kg/d (P < .01).

Conclusions: These data demonstrate that GLP-2 can enhance normal rat small intestine mucosal mass and absorption in vivo with the maximum effect seen at 50 μg/kg/d.

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