Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line

doi:10.1016/S0024-3205(97)00397-4

Life Sciences

Volume 61, Issue 4, 20 June 1997, Pages 403-409

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Abstract

Glutathione (GSH) is a tripeptide synthesised enzymatically from its components amino-acids by unicellular and multicellular organisms. GSH acts as a cellular anti-oxidant, protects the structural configuration of some enzymes, is involved in erythrocyte function and plays a role as co-enzyme in several reactions. We have found that GSH inhibits purified lactate dehydrogenase (1.56 U $LDH ml$ ) from rabbit skeletal muscle after 6 min pre-incubation with an ED₅₀ of about 5.4 μM. The inhibition is time dependent with a maximum after 45 minutes preincubation. Buffer (5 × 10⁻² M TRIZMA hydrochloride, pH 7.4) and a chelator (2 × 10⁻³ M EDTA) in the pre-incubation solution did not prevent the inhibition. Prolonged dialysis was almost without effect on GSH-inhibited LDH activity solution, indicating either an irreversible or a very tight binding inhibition. Kinetic analysis showed that this inhibition is of a very tight binding and at the same time of the uncompetitive type. GSH also inhibits LDH activity of rat M. soleus and M. gastrocnemius homogenates. This effect is probably unrelated to the reducing property of GSH since dithioerythritol (0.17–1.34 mM) does not mimic it. Loading of MDCK cells with glutathione ethylester completely prevented the acidification induced by 2,4-dinitrophenol, suggesting that GSH may influence the glycolytic pathway in vivo.

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Abstract

References (18)

Anal. Biochem.

Anal. Biochem.

Biochem. Pharmacol.

J. Appl. Physiol.

J Appl. Physiol.

Pflügers Archiv Europ J. Physiol.

Biochem. Int.

Enzymes

Cited by (4)

Ascorbic acid prolongs the viability and stability of isolated perfused lungs: A mechanistic study using <sup>31</sup>P and hyperpolarized <sup>13</sup>C nuclear magnetic resonance

Glutathione suppresses increase of serum creatine kinase in experimental hypoglycemia

Metabolic spectroscopy of inflammation in a bleomycin-induced lung injury model using hyperpolarized 1-<sup>13</sup>C pyruvate

Changes in hemolymph glutathione status after variation in photoperiod and light-irradiance in crayfish Procambarus clarkii and Procambarus digueti