[19] Antibodies that recognize nitrotyrosine
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Cited by (185)
Fenretinide favorably affects mucins (MUC5AC/MUC5B) and fatty acid imbalance in a manner mimicking CFTR-induced correction
2020, Biochimica et Biophysica Acta - Molecular and Cell Biology of LipidsDetection and quantification of nitric oxide-derived oxidants in biological systems
2019, Journal of Biological ChemistryCitation Excerpt :Poly- and monoclonal antibodies have been raised and are now commercially available. Immunohistochemical methods, immunoblotting techniques, and ELISAs have been developed and applied to the study of biological samples (184, 231–234). The typical readout of antibody-based methods is the difference in immunoreactivity in samples versus controls.
Peroxynitrite supports a metabolic reprogramming in merlin-deficient Schwann cells and promotes cell survival
2019, Journal of Biological ChemistryCitation Excerpt :VDAC (1:1,000, Cell Signaling Technology, catalog number 4866, RRID:AB_2272627); Hsp60 (1:1,000, Cell Signaling Technology, catalog number 12165, RRID:AB_2636980); complex IV (1;1,000, Cell Signaling Technology, catalog number 4850, RRID:AB_2085424); complex I (1:5,000, ThermoFisher Scientific, catalog number 459100, RRID:AB_2532223), cytochrome c (1:1,000, Cell Signaling Technology, catalog number 4280S, RRID:AB_10695410); pyruvate dehydrogenase (1:1,000, Cell Signaling Technology, catalog number 3205S, RRID:AB_2162926); SDHA (1:1,000, Cell Signaling Technology, catalog number 11998); Hsp90 (1:2,000, Santa Cruz Biotechnology, catalog number SC-7947); β-actin (1:30,000, Cell Signaling Technology, catalog number 3700, RRID:AB_2242334); α-tubulin (1:30,000, Cell Signaling Technology, catalog number 3873, RRID:AB_1904178); merlin (1:2,000, Cell Signaling Technology, catalog number 12888, RRID:AB_2650551); MnSOD (1:1,000, Millipore Sigma, catalog number 06-984); nNOS (Santa Cruz Biotechnology, catalog number sc-648, RRID:AB_630935); iNOS (Santa Cruz Biotechnology, catalog number sc-650, RRID:AB_631831); and eNOS (1:1,000, BD Biosciences, catalog number 610296, RRID:AB_397690). The mouse mAb against nitrated Hsp90 (1:2,000) was developed and characterized in-house (28), as well as the rabbit polyclonal antibody against nitrotyrosine (1:2,000) (70). Human Schwann cells mitochondrial content was assayed by measuring MitoTracker Green FM fluorescence in live cells according to the manufacturer's instructions (200 nm, Molecular Probes/ThermoFisher Scientific, catalog number M7514) using a Synergy H1 multi-mode reader (BioTek, Winooski, VT).