Trends in Pharmacological Sciences
ReviewAnalysis of exhaled breath condensate for monitoring airway inflammation
Section snippets
Collection of exhaled breath condensate
The collection of EBC samples is simple and easy to perform. In most studies, the equipment is home-made and generally consists of a mouthpiece with a one-way valve connected to a collecting system that is placed in either ice or liquid nitrogen to cool the breath [5]. The collecting system can be a condensing chamber with a double wall of glass, the inner wall of which is cooled by ice [7]. Generally, subjects wear a noseclip and are asked to breath tidally for 15 min through a mouthpiece
Markers in EBC
Several markers of oxidative stress and/or inflammatory mediators, such as 8-isoprostane [also known as 8-iso-prostaglandin F2α(PGF2α)], leukotrienes, prostaglandins, H2O2 and 3-nitrotyrosine, have been detected in EBC from healthy subjects and are increased in patients with different airway inflammatory diseases (Fig. 2). Collection of EBC is well accepted by patients, completely noninvasive and suitable for longitudinal studies. It is also possible to make measurements in children and in
Concluding remarks
Measurements of inflammatory mediators in EBC provide insights into the pathophysiology of inflammatory lung diseases. This method is: (1) completely noninvasive; (2) suitable for repeated measurements of lung inflammation during stable and/or exacerbated phases of airway diseases; and (3) potentially useful for assessing the efficacy of drug therapy. Analysis of EBC is a promising method to measure inflammatory mediators easily and could provide a novel, noninvasive approach to monitoring lung
Acknowledgements
This work was supported by the Catholic University of the Sacred Heart, grant'Research funds for the Faculty of Medicine', 2000–2001.
References (51)
- et al.
Exhaled markers of pulmonary disease
Am. J. Respir. Crit. Care Med.
(2001) Exhaled 8-isoprostane as an in vivo biomarker of lung oxidative stress in patients with COPD and healthy smokers
Am. J. Respir. Crit. Care Med.
(2000)Increased nitrotyrosine in exhaled breath condensate of patients with asthma
Am. J. Respir. Crit. Care Med.
(2000)- Montuschi, P. and Barnes, P.J. Exhaled leukotrienes and prostaglandins in asthma. J. Allergy Clin. Immunol. (in...
Increased levels of cysteinyl-leukotrienes in exhaled air condensate of aspirin-induced asthmatic patients
Am. J. Respir. Crit. Care Med.
(2001)Nitric oxide synthase inhibition decreases exhaled leukotriene B4 in patients with asthma
Am. J. Respir. Crit. Care Med.
(2001)Validation of a new technique to assess exhaled hydrogen peroxide: results from normals and COPD patients
Monaldi Arch. Chest Dis.
(2001)Catalase, myeloperoxidase and hydrogen peroxide in cystic fibrosis
Eur. Respir. J.
(1998)Hydrogen peroxide and nitric oxide in exhaled air of children with cystic fibrosis during antibiotic treatment
Eur. Respir. J.
(2000)Reactive nitrogen species and tyrosine nitration in the respiratory tract
Am. J. Respir. Crit. Care Med.
(1999)
Use of bronchoalveolar lavage in humans – past necessity and future imperative
Lung
Biopsy markers of airway inflammation and remodelling
Respir. Med.
Update on sputum methodology
Eur. Respir. J.
Role of exhaled nitric oxide in asthma
Immunol. Cell Biol.
Collection and analysis of exhaled breath condensate in humans
Am. J. Respir. Crit. Care Med.
Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients
Am. J. Respir. Crit. Care Med.
The isoprostanes in biology and medicine
Trends Endocrinol. Metab.
Exhaled breath condensate isoprostanes are elevated in patients with acute lung injury or ARDS
Chest
8-Epi-PGF2α, a novel noncyclooxygenase-derived prostaglandin, constricts airways in vitro
Am. J. Respir. Crit. Care Med.
Oxidative stress induced by acute ozone exposure is resistant to inhaled steroid treatment
Am. J. Respir. Crit. Care Med.
Exhaled 8-isoprostane as a new non-invasive biomarker of oxidative stress in cystic fibrosis
Thorax
Generation of the isoprostane 8-epi-prostaglandin F2α in vitro and in vivo via the cyclooxygenases
J. Pharmacol. Exp. Ther.
Non selective cyclo-oxygenase inhibition decreases exhaled prostaglandin E2 in patients with chronic obstructive pulmonary disease
Am. J. Respir. Crit. Care Med.
Chronic obstructive pulmonary disease is associated with an increase in urinary levels of isoprostane IPF2α-III, an index of oxidant stress
Am. J. Respir. Crit. Care Med.
Leukotrienes and inflammation
Am. J. Respir. Crit. Care Med.
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