Technological advances in high-throughput screening

https://doi.org/10.1016/S1367-5931(98)80089-6Get rights and content

Abstract

A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactions. Modifications have been developed for cell-free, homogeneous assay systems, such as time-resolved fluorescence, fluorescence polarization and the scintillation proximity assay. Innovations in engineering and chemistry have led to delivery systems for nanoliter volumes and sensitive biosensors for ultra-high-throughout screening conducted in nanoliter and picoliter volumes. Spectroscopic methods have been extended to read single molecule fluorescence. Technologies are being developed to identify new targets from genomic information in order to design the next generation of screens.

References (55)

  • R Seethala et al.

    A homogeneous, fluorescence polarization assay for Src-family tyrosine kinases (of outstanding interest)

    Anal Biochem

    (1997)
  • R Seethala et al.

    A fluorescence polarization competition immunoassay for tyrosine kinases (of special interest)

    Anal Biochem

    (1998)
  • GR Nakayama et al.

    A scintillating microplate assay for the assessment of protein kinase activity

    J Biomol Screening

    (1998)
  • DW Brandt

    Core system model: understanding the impact of reliability on high throughput screening systems (of special interest)

    Drug Discov Today

    (1998)
  • RW Wallace

    High throughput screening: Accessible UHTS?

    Drug Discov Today

    (1998)
  • C Shumate et al.

    Ultra-high throughput screening

    Lab Aut News

    (1997)
  • ES Razvi

    The current status of high throughput screening and outlook for the future, part 1 (of outstanding interest)

    Drug Market Dev

    (1998)
  • LJ Sannes

    Advances in high throughput screening (of outstanding interest)

    Spectrum: Drug Discovery and Design. Decision Resources

    (1997)
  • E Alderan et al.

    A novel approach to ultra-high-throughput screening

    Gen Eng News

    (1998)
  • B Haberman

    Genomics-based drug discovery: from gene sequencing to biology

    Decision Resources

    (1997)
  • A Persidis

    Proteomics

    Nat Biotechnol

    (1998)
  • C Ashton

    Proteomics — the new watchword for biotech (of outstanding interest)

    Scrip

    (1998)
  • S Fields

    The future is function

    Nat Genet

    (1997)
  • H Fei et al.

    Anatomic localization of alternatively spliced leptin receptors (Ob-R) in mouse brain and other tissues

    Proc Natl Acad Sci USA

    (1997)
  • A Persidis

    Lead validation platforms

    Nat Biotechnol

    (1998)
  • T Mistelli et al.

    Applications of green fluorescent protein in cell biology and biotechnology (of outstanding interest)

    Nat Biotechnol

    (1997)
  • RM Dickson et al.

    On/off blinking and switching behavior of single molecules of green fluorescent protein

    Nature

    (1997)
  • Cited by (0)

    View full text