Effect of capsaicin-evoked jaw-muscle pain on intramuscular blood-flow

doi:10.1016/j.archoralbio.2008.11.005

Archives of Oral Biology

Volume 54, Issue 3, March 2009, Pages 241-249

https://doi.org/10.1016/j.archoralbio.2008.11.005 Get rights and content

Abstract

Aim

To investigate effect of capsaicin-evoked masseter-muscle pain on intramuscular blood-flow (BF) at rest and during contractions.

Methods

Eight healthy men (22–31 years) participated. BF was measured with Laser Doppler (Moor Instruments, UK) using a single-fibre probe inserted into the right masseter. Three BF probes were attached to the skin above right and left masseter and the right-middle finger. Subjects performed 30 s isometric contractions at 5%, 15%, and 25% of maximal voluntary contraction. After the contractions, capsaicin (0.1 mL, 100 μg/mL) was injected into the right masseter close to the fibre probe. When the pain sensation had disappeared, the series of submaximal contractions were repeated. BF data were sampled continuously, processed in 10 s bins, and analyzed with repeated-measures ANOVAs.

Results

Intramuscular BF significantly increased immediately after capsaicin injection (P < 0.050) and rapidly (30 s) decreased to pre-injection values. A significant increase in cutaneous BF above the right masseter was observed (P < 0.050) and lasted for 10 min, while a significant BF decrease in the finger (P < 0.050) was observed. The contractions were associated with increases in intramuscular BF before and after the injection (P < 0.022) and the contraction levels were also associated with increase in intramuscular BF before injection (P = 0.008) but not after injection (P = 0.314).

Conclusions

This study demonstrated BF increased by muscle contraction but failed to show effects of contraction levels on BF in a muscle exposed to nociceptive stimuli. Neurogenic inflammation in muscles could possibly be mediated via antidromical effects and local release of vasoactive substances. The decreased BF in the finger could be due to involvement of central regulatory mechanisms.

Section snippets

Background

The most common complaint of patients with jaw-muscle disorders is a spontaneous deep ache often associated with tenderness to palpation or pain on movement.1, 2 Operationalized criteria for so-called myofascial temporomandibular pain (TMD) has been developed as described in the Research Diagnostic Criteria for TMD.³ Simons and Mense⁴ hypothesised that the compromised blood flow (BF) plays an important role in the development of muscle pain and tenderness subsequently leading to an energy

Subjects

Eight healthy men (mean age: 25.4 ± 3.7 years, range 22–31 years) participated in this study. All were University students and were in good health. The absence of painful muscle disorders and temporomandibular joint problems were checked in accordance with the Research Diagnostic Criteria (RDC) for TMD.³ Furthermore, none of the subjects took medication which could influence pain responses or vascular responses. Informed consent was obtained from each subject prior to inclusion, and the

Effect of intramuscular injections with the jaw at rest

The capsaicin injection evoked moderate pain which was rated as 37.5 ± 13.9 on the VAS 5 min after the injection and lasted for a total of 14.9 ± 1.4 min. In contrast, the injection of isotonic saline was associated with no or very low levels of pain (5.0 ± 10.0) and only lasted for 4.6 ± 1.7 min.

Immediately after the capsaicin and saline injections, BF in the right masseter muscle was significantly increased (Fig. 2A–D, Dunnett’s tests: P < 0.050). However, in both sessions the BF rapidly (about 30 s)

Discussion

This study demonstrated immediately after the capsaicin injection, cutaneous BF increases over the masseter muscle on the injection side, and cutaneous BF decreases in the middle finger with no BF changes in the contralateral cutaneous BF over the masseter muscle. The results also showed intramuscular BF increases evoked by submaximal levels of masseter muscle contraction but failed to show effects of contraction levels on intramuscular BF in a painful muscle. This study provides some

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Effect of capsaicin-evoked jaw-muscle pain on intramuscular blood-flow

Abstract

Aim

Methods

Results

Conclusions

Section snippets

Background

Subjects

Effect of intramuscular injections with the jaw at rest

Discussion

Pain

Arch Oral Biol

Clin Neurophysiol

Brain Res

Pain

J Therm Biol

Pain

Brain Res

Prog Brain Res

Brain Res

Eur J Pain

Arch Oral Biol

Arch Oral Biol

Masticatory muscle disorders. Temporomandibular joint and masticatory muscle disorders

Signs and symptoms of temporomandibular disorders. Management of temporomandibular disorders and occlusion

Research diagnostic criteria for temporomandibular disorders: review, criteria, examinations and specifications, critique

J Craniomandib Disord Facial Oral Pain

Mechanism of ischemic pain in human muscles of mastication: intramuscular pressure, EMG, force and blood flow of the temporal and masseter muscles during biting

Adv Pain Res Ther

Contraction frequency, blood supply, and muscle pain

J Appl Physiol

Local muscle blood flow and sustained contractions of human arm and back muscles

Eur J Appl Physiol Occup Physiol

Skeletal muscle tension, flow, pressure, and EMG during sustained isometric contractions in humans

Eur J Appl Physiol Occup Physiol

Plasticity in corticomotor control of the human tongue musculature induced by tongue-task training

Exp Brain Res

One hour of tongue-task training is associated with plasticity in corticomotor control of the human tongue musculature

Exp Brain Res

Effects of muscle fatigue induced by low-level clenching on experimental muscle pain and resting jaw muscle activity: gender differences

Exp Brain Res

A pharmacological approach to elucidation of the role of different nerve fibres and receptor endings in mediation of pain

J Physiol (Paris)

Neurogenic hyperalgesia: the search for the primary cutaneous afferent fibers that contribute to capsaicin-induced pain and hyperalgesia

J Neurophysiol

Neurogenic hyperalgesia: psychophysical studies of underlying mechanisms

J Neurophysiol