New technologyIn Vivo Evaluation of a Porous, Elastic, Biodegradable Patch for Reconstructive Cardiac ProceduresDisclaimer
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Experimental Animals
Adult male syngeneic Lewis rats (Harlan Sprague Dawley Inc, Indianapolis, IN) weighing 300 g to 350 g were used for the RVOT replacement procedure. The research protocol followed the National Institutes of Health guidelines for animal care and was approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh.
Material Synthesis and Preparation
The PEUU was synthesized from butyl diisocyanate, polycaprolactone (2,000 MW), and putrescine [3] and processed according to the methods of previous reports in
Postoperative Course and Gross Observations
No deaths occurred during the postoperative course in either group and no gross evidence of thrombosis was present in any of the animal explants. At the time of explantation, all rat hearts exhibited minimal thoracic adhesions with no recognizable pattern of adhesive tissue present in ePTFE or PEUU implanted animals. Neither group showed any dehiscence or aneurysm formation at the site of the implanted patch in the RVOT at each time point. Looking at macroscopic images of the RVOT 12 weeks
Comment
The results with the PEUU patch represent the first report on the implantation of this material as a step toward more extensive in vivo studies in the cardiovascular system. This material has theoretical advantages over nondegradable materials used in reconstructive cardiovascular procedures in that it seems capable of mechanically performing early in the implant period while allowing tissue ingrowth that takes over this mechanical role by 3 months in this model. As with other
Disclosures and Freedom of Investigation
This work was supported by the National Institutes of Health (HL #069368). The polyester urethane urea material was synthesized and processed by the authors, who also had full control of the study design, methods used, outcome measurements, data analysis, and production of the written report.
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The Society of Thoracic Surgeons, the Southern Thoracic Surgical Association, and The Annals of Thoracic Surgery neither endorse nor discourage use of the new technology described in this article.