Biochemical and Biophysical Research Communications
Critical role of heme oxygenase-1 in Foxp3-mediated immune suppression
Section snippets
Materials and methods
Reagents and media. RPMI 1640 supplemented with 2 mM l-glutamine, 1% nonessential amino acids, 1% pyruvate, 100 U/mL penicillin, 100 μg/mL streptomycin (Life Technologies, Grand Island, NY), and 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT) was used as complete medium in all cultures. Anit-human CD3, anti-human CD28, and zinc protoporphyrin were purchased from BD Biosciences (Mountain View, CA) and Porphyrin Products (Logan, UT), respectively. Concanavalin A (Con
Induction of HO-1 expression by Foxp3 gene transfection
Jurkat T cells were transfected with Foxp3 gene, and the gene expression was confirmed by Western blot analysis (Fig. 1A). To characterize the properties of Foxp3-transfected Jurkat T cells, we assessed the production of cytokines and proliferative responses in the cells transfected with Foxp3 gene or with control gene. Upon stimulation with PMA and ionomycin, the productions of IL-2 and IFN-γ was significantly reduced in Foxp3-transfected Jurkat T cells, as compared with the cells transfected
Discussion
Identification of signals that regulate the proliferation and function of Treg is of great importance to understand the mechanisms by which the immune tolerance breaks down and the autoimmunity develops. In the present study, we have found that Foxp3 transfer into T cells can induce HO-1 expression, which is causally associated with Foxp3-mediated development of T cells with regulatory functions.
Given that Foxp3 is a highly reliable marker for Treg cells, a key issue then in defining Treg cells
Acknowledgment
This work was supported by a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare (01-PJ3-PG6-01GN09-003).
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