Biochemical and Biophysical Research Communications
Hedgehog is involved in prostate basal cell hyperplasia formation and its progressing towards tumorigenesis
Section snippets
Materials and methods
Human tissues. The human prostate tissues were obtained from 42 patients who had received radical prostectomy at Department of Pathology, Chung Shan Medical University, Taichung, Taiwan, or Department of Urology, Changhua Christian Hospital, Changhua, Taiwan. The presence of normal basal cell layer (NBCL), basal cell hyperplasia (BCH), and prostate cancer (CaP) was confirmed by clinical pathologists. Tissue donors were informed with written consents before obtainment of their prostate
Dynamic p63 expression during transformation of normal basal cell layer towards hyperplasia and cancer
Taking advantage of the heterogeneity of human prostate tumors, we examined the dynamic changes of p63 expression during the transformation of NBCL to BCH and further towards CaP. Because p63 had been widely used as a basal cell marker, we used p63 to define BCH, irrespective of whether the specimen had been diagnosed as CaP or non-CaP. As such, BCH was used instead of the generally used benign prostatic hyperplasia (BPH). In the normal prostate sites, p63 expression was detected in the slim
Acknowledgments
This study was supported by grants from Department of Heath (DOH 95-TD-B-111-TM009), Taiwan to D.P.C.L. and from National Science Council (NSC 95-2320-B-040-035), Taiwan to H.H.C.
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