Biochemical and Biophysical Research Communications
Ionizing irradiation induces apoptotic damage of salivary gland acinar cells via NADPH oxidase 1-dependent superoxide generation
Section snippets
Materials and methods
Cell lines. Immortalized normal salivary gland (NS-SV-AC and NS-SV-DC) cell lines were provided by Dr. M. Azuma (Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokushima University) and were maintained in keratinocyte basal medium-2 (KBM-2, Cambrex Bio Science Inc., Walkersville, MD) [12].
MTT assay. The antiproliferative effects of radiation were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye uptake method [13].
Reverse
The sensitivity of NS-SV-AC and NS-SV-DC cells to γ-rays
When NS-SV-AC cells were exposed to a single dose irradiation of 2–10 Gy, their growth was inhibited in a dose-dependent manner and the growth inhibition was approximately 50% of the control level after 10 Gy irradiation. In NS-SV-DC cells, the growth inhibition was approximately 15% after 2–10 Gy irradiation, indicating that NS-SV-DC cells were rather nonsensitive to γ-rays compared with NS-SV-AC cells (Fig. 1A). Apoptosis in the irradiated NS-SV-AC cells was increased in a time-dependent manner
Discussion
Along with studies on ROS generation, increasing studies of the role of Nox family proteins have been reported. However, the involvement of ROS and Nox family proteins in the impairment of the salivary glands has not been sufficiently clarified. There are several causes of salivary gland dysfunction, including aging, medications, radiotherapy for cancers of the head and neck, and serious salivary gland disorders. The iatrogenic (cancer treatment-induced) hypofunction of the salivary glands has
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