Reviews and feature articleIL-17 in atopic eczema: Linking allergen-specific adaptive and microbial-triggered innate immune response
Section snippets
Patients
Patients with AE according to the criteria of Hanifin and Rajka (n = 3) and a positive skin prick test response to Dermatophagoides pteronyssinus, RAST class of 3 or greater to Der p 1, and positive APT reaction to D pteronyssinus were included in the study. Before blood or skin samples were obtained, each participant provided informed consent. The study was approved by the ethics committee of the Istituto Dermopatico Dell'Immacolata.
Cytokines and antibodies
The following antibodies were used in flow cytometric
IL-17–producing T lymphocytes infiltrate the skin during APT reactions: Newly characterized TH2/IL-17 subset
Three patients with AE with a documented hypersensitivity to D pteronyssinus were challenged with APTs to D pteronyssinus. Biopsy specimens were taken from the resulting eczematous reactions, and infiltrating T cells were isolated and characterized by means of intracellular cytokine staining with flow cytometric techniques. In line with the hypothesis of a type 2 domination in early AE, the majority of skin-derived T cells activated in vitro by PMA plus ionomycin expressed high levels of IL-4 (
Discussion
Increasing evidence suggests a central role of IL-17 in encompassing host defense against microorganisms at surface barriers. In patients with AE, disease severity positively correlates with skin colonization of S aureus. This observation led us to investigate IL-17 in the pathogenesis of AE.
In this study we demonstrate that distinct subpopulations of IL-17–secreting T cells infiltrate acute skin lesions, where they trigger keratinocytes to produce the antimicrobial peptide HBD-2. However, this
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K. Eyerich was supported by a grant of the “Bayerische Forschungsstiftung” (BFS). This work was supported by a grant of the European Community (UE-LSHB-CT-2005-018681) and by the Italian minister of health.
Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest.
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These authors contributed equally to this work.