Invited review
The pilocarpine model of temporal lobe epilepsy

https://doi.org/10.1016/j.jneumeth.2008.04.019Get rights and content
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Abstract

Understanding the pathophysiogenesis of temporal lobe epilepsy (TLE) largely rests on the use of models of status epilepticus (SE), as in the case of the pilocarpine model. The main features of TLE are: (i) epileptic foci in the limbic system; (ii) an “initial precipitating injury”; (iii) the so-called “latent period”; and (iv) the presence of hippocampal sclerosis leading to reorganization of neuronal networks. Many of these characteristics can be reproduced in rodents by systemic injection of pilocarpine; in this animal model, SE is followed by a latent period and later by the appearance of spontaneous recurrent seizures (SRSs). These processes are, however, influenced by experimental conditions such as rodent species, strain, gender, age, doses and routes of pilocarpine administration, as well as combinations with other drugs administered before and/or after SE. In the attempt to limit these sources of variability, we evaluated the methodological procedures used by several investigators in the pilocarpine model; in particular, we have focused on the behavioural, electrophysiological and histopathological findings obtained with different protocols. We addressed the various experimental approaches published to date, by comparing mortality rates, onset of SRSs, neuronal damage, and network reorganization. Based on the evidence reviewed here, we propose that the pilocarpine model can be a valuable tool to investigate the mechanisms involved in TLE, and even more so when standardized to reduce mortality at the time of pilocarpine injection, differences in latent period duration, variability in the lesion extent, and SRS frequency.

Abbreviations

AEDs
antiepileptic drugs
CA
Cornu Ammonis
EEG
electroencephalogram
i.p.
intraperitoneal
MRI
magnetic resonance imaging
P
postnatal
s.c.
subcutaneous
SE
status epilepticus
SRSs
spontaneous recurrent seizures
TLE
temporal lobe epilepsy

Keywords

Animal models
Entorhinal cortex
Hippocampus
Pilocarpine
Temporal lobe epilepsy

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