NeuroanatomyDistribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus
Section snippets
Animals and tissue preparation
Tissue was obtained from rats (Sprague–Dawley; Charles River, Wilmington, MA, USA) using experimental procedures consistent with those outlined in National Institutes of Health publication 91–3207, Preparation and Maintenance of Higher Animals During Neuroscience Experiments, and approved by the Institutional Animal Care and Use Committee at the Yale University School of Medicine. Every effort was made to minimize the number of animals used and their suffering. Animals were deeply anesthetized
General InsP3R isotype 1–3 immunoreactivity in mature rat hippocampus
Consistent with previous reports (Nakanishi et al 1991, Sharp et al 1993, Sharp et al 1999, Dent et al 1996), InsP3R1 immunoreactivity was found throughout the mature rat hippocampus (>8 weeks old) with the strongest labeling in area CA1 (Figs. 1A and 2A). InsP3R1 immunostaining appeared to be exclusively located in neurons, while InsP3R2 immunoreactivity was only found in a subpopulation of astrocytes (Sharp et al., 1999) and in endothelial cells of capillaries (Fig. 1B). Fine astrocytic
Discussion
In this study we examined the distribution of the intracellular Ca2+ channel proteins InsP3R1-3 and RyR1-3 in the hippocampus of maturing rat. The greatest changes occurred with type 1 InsP3Rs. Because synaptically elicited internal Ca2+ release in hippocampal pyramidal neurons has been reported to depend exclusively on InsP3Rs (Pozzo-Miller et al 1996, Nakamura et al 1999, Kapur et al 2001), we focused our analysis on this protein. Our principal novel findings are the following: (1) during
Acknowledgments
We gratefully acknowledge Constantinos Paspalas for his generous help with this project and for his insightful comments on this manuscript. We thank Rick Matthews for use of his confocal microscope. We also thank John Fitzpatrick for writing our computer analysis software, Anna Hagenston for a critical reading of the manuscript, Amanda Sleeper for her initial help with the experiments, and Keith Gipson for many thoughtful discussions. This research was supported by Whitehall Foundation, Kavli
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Present address: Department of Neurosurgery Ruprecht-Karls-University Heidelberg, Germany.