Rapid reportEstrogen receptor α is expressed on the cell-surface of embryonic hypothalamic neurons
Section snippets
Experimental procedures
All experimental procedures were approved by the local Committee on Bioethics and followed the NIH Guidelines for the Care and Use of Laboratory Animals. All efforts were made to minimize both the suffering and the number of animals used. Unless otherwise specified, all drugs were from Sigma-Aldrich, St. Louis, MO, USA.
Identification of membrane ER from hypothalamic tissue
To determine the presence of ERs at the plasma membrane of E16 hypothalamic tissue we used sub-cellular fractionation and immunoblotting. The plasma membrane fraction was isolated using Percoll gradient centrifugation; absence of nuclear and cytosolic contamination was corroborated with anti-histone H1 and anti-LIMK-1 antibodies, respectively. As membrane purification control, incubation with a specific anti-TrkB revealed, as expected, a 145 kDa band corresponding to membrane receptor. Results
Conclusion
In summary, we have identified an ERα variant in the plasmalemmal fraction of hypothalamic tissue and on the cell-surface of hypothalamic neurons in cultures. We have also shown that the mERα is under E2-regulation and mediates E2 induced-ERK phosphorylation in a dose-dependent manner and in a time-course compatible with a membrane effect rather than a genomic action. Although the mechanisms of E2 translocation to the neuronal cell-surface will have to be addressed, the evidence presented in
Acknowledgments
This work was supported by grants from CONICET-PIP 6238 (M.J.C.) and ANPCyT-PICT 05-26331 (M.J.C.) and PICT 05-1429 (A.G.L.). S.V.G. is a CONICET fellow; A.G.L. and M.J.C. are career members of CONICET. We thank Dr. L. Heredia for technical advice on biotinylation experiments. M.J.C. is also indebted to Dr. H. F. Carrer for helpful discussions and for critical reading of the manuscript.
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