Islet transplantation: outcomeHuman islet graft function in NOD-SCID mice predicts clinical response in islet transplant recipients☆
Section snippets
Methods
Pancreata were obtained from heart-beating, cadaveric, multiorgan donors. Isolations were performed using our modification of the Ricordi technique, which was previously published.6 To test the islet function and the viability in three human islet transplants performed using the Edmonton protocol,7 we transplanted an aliquot of 2000 IEQ from each islet preparation under each kidney capsule of groups of 10 NOD-SCID mice. Following an overnight fast, NOD-SCID mice were injected intraperitoneally
Results
The IEQ of each human islet transplantation is listed in Table 1. Both C-peptide and insulin production patterns in the mice as shown in Fig 1 correlated with the clinical course of the patients.
Conclusion
The in vivo mouse assay is a good surrogate marker for islet potency and function in humans. Thus, the assay can be used to screen preparations for their suitability for transplantation after being cultured.
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Cited by (0)
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This work is supported in part by USPHS/NIH Grant DK57700, USPHS/NCRR Grant RR16602, Assisi Foundation, and Juvenile Diabetes Research Foundation Grant 1-2000-416 to Dr A.O. Gaber.