Abstract
Human serum albumin (HSA) nanospheres with a size less than 200 nm in diameter were prepared using a modified coacervation method and crosslinking with methyl polyethylene glycol modified oxidized Dextram (Dextranox-MPEG) which created a sterically stabilizing polyethylene oxide surface layer surrounding the nanospheres. The crosslinking efficiency and the surface characteristics of glutaraldehyde and Dextranox-MPEG crosslinked HSA nanospheres were determined and compared. The zeta potential of the Dextranox-MPEG crosslinked particles was significantly lower than that of glutaraldehyde stabilized particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by an electrolyte and pH induced flocculation test. The Dextranox-MPEG crosslinked nanospheres showed a significantly reduced plasma protein adsorption on the particle surface compared with glutaraldehyde crosslinked nanospheres.
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Lin, W., Coombes, A.G.A., Garnett, M.C. et al. Preparation of Sterically Stabilized Human Serum Albumin Nanospheres Using a Novel Dextranox-MPEG Crosslinking Agent. Pharm Res 11, 1588–1592 (1994). https://doi.org/10.1023/A:1018957704209
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DOI: https://doi.org/10.1023/A:1018957704209