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Induction of Microsomal Prostaglandin E Synthase-1 in Human Gingival Fibroblasts

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Abstract

It is well established that prostaglandin E2 (PGE2) plays an important role in inflammatory diseases including periodontitis. Previously we have reported that the inflammatory mediators interleukin-1β (IL-1β) and tumor necrosis factor α (TNFα ) stimulate PGE2 synthesis by inducing mRNA expression of cyclooxygenase-2 (COX-2) in human gingival fibroblasts. In present study the involvement of microsomal prostaglandin E synthase-1 (mPGES-1) in relation to PGE2 production was investigated. The results showed that IL-1β as well as TNFα induced mPGES-1 mRNA and protein expression accompanied by enhanced PGE2 production in gingival fibroblasts. The anti-inflammatory steroid dexamethasone (DEX) inhibited mPGES-1 mRNA and protein expression as well as PGE2 production induced by IL-1β or TNFα. The COX-2 specific inhibitor, celecoxib, in contrast to the nonspecific COX inhibitor, indomethacin, markedly reduced mPGES-1 expression induced by IL-1β. The results demonstrate that mPGES-1 regulates PGE2 production in gingival fibroblasts stimulated by inflammatory mediators IL-1β and TNFα. This novel pathway may be a potential target for treatment strategies of periodontal disease.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, Institute of Odontology, Karolinska Institutet, Huddinge, Sweden

    Tülay Yucel-Lindberg, Therese Hallström, Anna Kats, Manal Mustafa & Thomas Modéer

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  1. Tülay Yucel-Lindberg
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  2. Therese Hallström
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Correspondence to Tülay Yucel-Lindberg.

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Yucel-Lindberg, T., Hallström, T., Kats, A. et al. Induction of Microsomal Prostaglandin E Synthase-1 in Human Gingival Fibroblasts. Inflammation 28, 89–95 (2004). https://doi.org/10.1023/B:IFLA.0000033024.13748.c1

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