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Differential effects of cyclic AMP on the in vitro induction of antibody synthesis

Abstract

INTRACELLULAR events in lymphocytes following antigenic stimulation still remain largely obscure. It has recently been demonstrated that cyclic AMP is involved during early events of the in vitro immune induction, since exogenous application of cyclic AMP can prevent spleen lymphocytes from differentiating into antibody-producing cells1. One can assume that this is more than a general effect on cell division processes, since inhibition by cyclic AMP could be demonstrated only during the first 24 h of the immune induction period, whereas cell proliferation starts after 24 h (ref. 2). Since cooperation between T and B cells and proliferation as well as differentiation of these cells are essential features during the early period of the immune response against most antigens3 it was of interest to study the effect of cyclic AMP on the primary immune response in a T-cell–deficient system such as nude spleen cell cultures. Nude spleen cells can be stimulated to antibody production against sheep red blood cells (SRBC) not only in the presence of allogeneic T cells and antigen4,5 but also by B cell mitogens, such as lipopolysaccharides (LPS) from various bacteria without a specific antigen6.

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BÖSING-SCHNEIDER, R. Differential effects of cyclic AMP on the in vitro induction of antibody synthesis. Nature 256, 137–138 (1975). https://doi.org/10.1038/256137a0

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