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Specific binding of 3H-mepyramine to histamine H1 receptors in intestinal smooth muscle

Abstract

THE specific binding of high-affinity ligands to a wide range of pharmacological receptors has been used to obtain information on receptor numbers, distribution and the characteristics of agonist and antagonist binding. The first study of this type, by Paton and Rang1, was of the binding of 3H-atropine to muscarinic receptors in guinea pig intestinal smooth muscle. The histamine H1 receptor has not previously been studied, and here we describe the specific binding of mepyramine, an H1-selective antagonist2, to histamine H1 receptors in homogenates of the longitudinal muscle-myenteric plexus preparation from guinea pig small intestine.

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References

  1. Paton, W. D. M. & Rang, H. P. Proc. R. Soc. B 163, 1–44 (1965).

    Article  ADS  CAS  Google Scholar 

  2. Ash, A. S. F. & Schild, H. O. Br. J. Pharmac. Chemother. 27, 427–439 (1966).

    Article  CAS  Google Scholar 

  3. Marshall, P. B. Br. J. Pharmac. Chemother. 10, 270–278 (1955).

    Article  CAS  Google Scholar 

  4. Arunlakshana, O. & Schild, H. O. Br. J. Pharmac.Chemother. 14, 48–58 (1959).

    Article  CAS  Google Scholar 

  5. Wilkinson, G. N. Biochem. J. 80, 324–332 (1961).

    Article  CAS  Google Scholar 

  6. Reasbeck, P. G. & Young, J. M. Br. J. Pharmac. 48, 148–155 (1973).

    Article  CAS  Google Scholar 

  7. Paton, W. D. M. & Rang, H. P. Adv. Drug Res. 3, 57–80 (1966).

    Google Scholar 

  8. Black, J. W., Duncan, A. M., Durant, C. J., Ganellin, C. R. & Parsons, E. M. Nature 236, 385–390 (1972).

    Article  ADS  CAS  Google Scholar 

  9. Green, A. F. Br. J. Pharmac. Chemother. 8, 171–176 (1953).

    Article  CAS  Google Scholar 

  10. Rang, H. P. Br. J. Pharmac. Chemother. 22, 356–365 (1964).

    Article  CAS  Google Scholar 

  11. Fewtrell, C. M. S. & Rang, H. P. in Drug Receptors (ed. Rang, H. P.) 211–224 (Macmillan, London, 1973).

    Book  Google Scholar 

  12. Burgen, A. S. V., Hiley, C. R. & Young, J. M. Br. J. Pharmac. 50, 145–151 (1974).

    Article  CAS  Google Scholar 

  13. Yamamura, H. I. & Snyder, S. H. Molec. Pharmac. 10, 861–867 (1974).

    CAS  Google Scholar 

  14. Yamamura, H. I. & Snyder, S. H. Proc. natn. Acad. Sci. U.S.A. 71, 1725–1729 (1974).

    Article  ADS  CAS  Google Scholar 

  15. Birdsall, N. J. M., Burgen, A. S. V., Hulme, E. C. & Wells, J. W. Br. J. Pharmac. 59, 503P (1977).

    CAS  Google Scholar 

  16. Takagi, K. & Uchida, M. Jap. J. Pharmac. 20, 272–286 (1970).

    Article  CAS  Google Scholar 

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HILL, S., YOUNG, J. & MARRIAN, D. Specific binding of 3H-mepyramine to histamine H1 receptors in intestinal smooth muscle. Nature 270, 361–363 (1977). https://doi.org/10.1038/270361a0

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