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Hamster preproglucagon contains the sequence of glucagon and two related peptides

Abstract

Glucagon is a 29-amino acid polypeptide hormone synthesized by the A cells of the endocrine pancreas1–4. Its primary site of action is the liver where it stimulates glycogenolysis, gluconeogenesis and ketogenesis. In mammals, biosynthetic studies have shown that glucagon is derived from a precursor of molecular weight (Mr) approximately 18,000 which is five to six times larger than glucagon5. Glucagon-containing polypeptides and immunoreactants of various sizes have also been described from stomach, intestine, brain and salivary gland3. Here, we have determined the structure of hamster pancreatic preproglucagon from the sequence of its cDNA. This 180-amino acid precursor contains the sequence of glucagon and two glucagon-like polypeptides arranged in tandem. The precursor also contains the sequences of several non-pancreatic glucagon-containing polypeptides which suggests that, in mammals, both pancreatic and non-pancreatic glucagon and glucagon-containing polypeptides may be derived from a common precursor by tissue-specific processing. We have tentatively identified each of the glucagon-like immunoreactants which have been described with respect to the sequence of proglucagon and have proposed a scheme for the processing of pancreatic proglucagon.

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References

  1. Foa, P., Bajaj, J. & Foa, N. (eds) Glucagon: Its Role in Physiology and Clinical Medicine (Springer, Berlin, 1977).

    Google Scholar 

  2. Unger, R.H. & Orei, L. (eds) Glucagon. Physiology, Pathophysiology and Morphology of the Pancreatic A-Cell (Elsevier, New York, 1981).

  3. Conlon, J. M. Diabetologia 18, 85–88 (1980).

    Article  CAS  Google Scholar 

  4. Unger, R. H. & Orci, L. New Engl J. Med. 304, 1518–1524 (1981).

    Article  CAS  Google Scholar 

  5. Patzelt, C., Tager, H. S., Carroll, R. S. & Steiner, D. F. Nature 282, 260–266 (1979).

    Article  ADS  CAS  Google Scholar 

  6. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  Google Scholar 

  7. Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).

    Article  CAS  Google Scholar 

  8. Carmichael, G. C. & McMaster, G. K. Meth. Enzym. 65, 380–391 (1980).

    Article  CAS  Google Scholar 

  9. Thomas, P. S. Proc. natn. Acad. sci. U.S.A. 77, 5201–5205 (1980).

    Article  ADS  CAS  Google Scholar 

  10. Thim, L. & Moody, A. J. Regulatory Peptides 2, 139–150 (1981).

    Article  CAS  Google Scholar 

  11. Moody, A. J., Hoist, J. J., Thim, L. & Lindkaer Jenson, S. Nature 289, 514–516 (1981).

    Article  ADS  CAS  Google Scholar 

  12. Tager, H. S. & Steiner, D. F. Proc. natn. Acad. sci. U.S.A. 70, 2321–2325 (1973).

    Article  ADS  CAS  Google Scholar 

  13. Bataille, D. et al. FEBS Lett. 146, 79–86 (1982).

    Article  CAS  Google Scholar 

  14. Lund, P. K., Goodman, R. H., Dee, P. C. & Habener, J. F. Proc. natn. Acad. sci. U.S.A. 79, 345–349 (1982).

    Article  ADS  CAS  Google Scholar 

  15. Shen, L. P., Pictet, R. L. & Rutter, W.J. Proc. natn. Acad. sci. U.S.A. 79, 4575–4579 (1982).

    Article  ADS  CAS  Google Scholar 

  16. Chan, S. J. et al. J. biol. Chem. 256, 7595–7602 (1981).

    CAS  PubMed  Google Scholar 

  17. Lund, P. K., Goodman, R.H. & Habener, J.F. J. biol. Chem. 256, 6515–6518 (1981).

    CAS  PubMed  Google Scholar 

  18. Shields, D., Warren, T. G., Roth, S. E. & Brenner, M. J. Nature 289, 511–514 (1981).

    Article  ADS  CAS  Google Scholar 

  19. Steiner, D. F., Quinn, P. S., Chan, S. J., Marsh, J. & Tager, H.S. Ann. N.Y. Acad. sci. 343, 1–16 (1980).

    Article  ADS  CAS  Google Scholar 

  20. Tager, H. S. & Markese, J. J. biol. Chem. 254, 2229–2233 (1979).

    CAS  Google Scholar 

  21. Kirkegaard, P. et al. Nature 297, 156–157 (1982).

    Article  ADS  CAS  Google Scholar 

  22. Lerner, R. A. Nature 299, 592–596 (1982).

    Article  ADS  CAS  Google Scholar 

  23. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5299 (1979).

    Article  CAS  Google Scholar 

  24. Santerre, R. F. et al. Proc. natn. Acad. sci. U.S.A. 78, 4339–4343 (1981).

    Article  ADS  CAS  Google Scholar 

  25. Land, H., Grez, M., Hauser, H., Lindenmaier, W. & Schutz, G. Nucleic Acids Res. 9, 2251–2266 (1981).

    Article  CAS  Google Scholar 

  26. Villa-Komaroff, L. et al. Proc. natn. Acad. sci. U.S.A. 75, 3727–3731 (1978).

    Article  ADS  CAS  Google Scholar 

  27. Gergen, J. P., Stern, R. H. & Wensink, P. C. Nucleic Acids Res. 7, 2115–2136 (1979).

    Article  CAS  Google Scholar 

  28. Comb, M., Seeburg, P. H., Adelman, J., Eiden, L. & Herbert, E. Nature 295, 663–666 (1982).

    Article  ADS  CAS  Google Scholar 

  29. Beaucage, S. L. & Caruthers, M. H. Tetrahedron Lett. 22, 1859–1862 (1981).

    Article  CAS  Google Scholar 

  30. Bell, G. I. et al. Nature 282, 525–527 (1979).

    Article  ADS  CAS  Google Scholar 

  31. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  32. Dayhoff, M. O. Atlas of Protein Sequence and Structure Vol. 5, Suppl. 2, 125–126 (National Biomedical Research Foundation, Washington, DC, 1976).

    Google Scholar 

  33. Tatemoto, K. & Mutt, V. Proc. natn. Acad. sci. U.S.A. 78, 6603–6607 (1981).

    Article  ADS  CAS  Google Scholar 

  34. Jornval, H. et al. FEBS Lett. 123, 205–216 (1981).

    Article  Google Scholar 

  35. Guillemin, R. et al. Science 218, 585–587 (1982).

    Article  ADS  CAS  Google Scholar 

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Bell, G., Santerre, R. & Mullenbach, G. Hamster preproglucagon contains the sequence of glucagon and two related peptides. Nature 302, 716–718 (1983). https://doi.org/10.1038/302716a0

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