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Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family

Abstract

Amino-acid sequences derived from complementary DMAs encoding the α- and β-subunits of the GAB A/ benzo diazepine receptor from bovine brain show homology with other ligand-gated receptor subunits, suggesting that there is a super-family of ion-channel-containing receptors. Co-expression of the in vitro-generated α-subunit and β-subunit RNAs in Xenopus oocytes produces a functional receptor and ion channel with the pharmacological properties characteristic of the GABAA receptor.

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References

  1. 1. Olsen, R. W. & Venter, J. C. (eds) Benzodiazepine /GABA Receptors and Chloride Channels: Structural and Functional Properties (Liss, New York, 1986). 2. Turner, A. J. & Whittle, S. R. Biochem. J. 209, 29–41 (1983). 3. Sigel, E., Stephenson, F. A., Mamalaki, C. & Barnard, E. A. J. biol. Chem. 258, 6965–6971 (1983). 4. Sigel, E. & Barnard, E. A. / biol. Chem. 259, 7219–7223 (1984). 5. Casalotti, S. O., Stephenson, F. A. & Barnard, E. A. /. biol. Chem. 261, 15013–15016 (1986). 6. Mamalaki, C., Stephenson, F. A. & Barnard, E. A. EMBO J. 6, 561–565 (1987). 7. Schoch, P. et al. Nature 314, 168–171 (1985). 8. Huynh, T. V., Young, R. A. & Davis, R. W. in: DNA Cloning: A Practical Approach, Vol. 1 (ed. Glover, D. M.) 49–78 (IRL, Oxford, 1985). 9. Von Heijne, G., Nucleic Acids Res. 14, 4683–4690 (1986). 10. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982). 11. Schwartz, R. M. & Dayhoff, M. O. in Atlas of Protein Sequence and Structure, Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 353–358 (Nat. Biomed. Res. Fdn., Washington DC, 1978). 12. Sweetnam, P. M. & Tallman, J. F. Molec. Pharmacol. 29, 299–306 (1986). 13. Feramisco, J. R., Glass, D. B. & Krebs, E. G. / biol. Chem. 255, 4240–4245 (1980). 14. Kubo, T. et al. Eur. J. Biochem. 149, 5–13 (1985). 15. Popot, J–L. & Changeux, J–P. Physiol. Rev. 64, 1162–1239 (1984). 16. Criado, M., Sarin, V., Fox, J. L. & Lindstrom, J. Biochemistry 25, 2839–2846 (1986). 17. Kao, P. N. & Karlin, A. /. biol. Chem. 261, 8085–8088 (1986). 18. Goldman, D. et al. Cell 48, 965–973 (1987). 19. Conti–Tronconi, B. M., Hunkapiller, M. W. & Raftery, M. A. Proc. natn. Acad. Sci. U.S.A. 81, 2631–2634 (1984). 20. Hermans–Borgmeyer, L et al. EMBO J. 5, 1503–1508 (1986). 21. Hucho, F., Oberthur, W. & Lottspeich, F. FEBS Lett. 205, 137–142 (1986). 22. Giraudat, J., Dennis, M., Heidmann, T., Chang, J.–Y. & Chargeux, J.–P. Proc. natn. Acad. Sci. U.S.A. 83, 2719–2723 (1986). 23. Imoto, K. et al. Nature 324, 670–674 (1986). 24. McCarthy, M. P. et al. A. Rev. Neurosci. 9, 383–413 (1986). 25. Grenningloh, G. et al. Nature 328, 215–220 (1987). 26. Ono, J. K. & Salvaterra, P. M. J. Neurosci. 1, 259–270 (1981). 27. Dixon, R. A. F. et al. Nature 321, 75–79 (1986). 28. Kubo, T. et al. Nature 323, 411–416 (1986). 29. Nathans, J. & Hogness, D. S. Cell 34, 807–814 (1983). 30. Sumikawa, K., Houghton, M., Emtage, J. S., Richards, B. M. & Barnard, E. A. Nature 292, 862–864 (1981). 31. Barnard, E. A., Miledi, R. & Sumikawa, K. Proc. R. Soc. Lond. B 215, 241–246 (1982). 32. Smart, T. G., Constanti, A., Bilbe, G., Brown, D. A. & Barnard, E. A. Neurosci. Lett. 40, 55–59 (1983). 33. Houamed, K. M. et al. Nature 310, 318–321 (1984). 34. Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984). 35. Dascal, N., Landau, E. M. & Lass, Y. / Physiol. 352, 551–574 (1984). 36. Study, R. E. & Barker, J. L. Proc. natn. Acad. Sci. U.S.A. 78, 7180–7184 (1981). 37. Bormann, J., Hammill, O. P. & Sakmann, B. J. Physiol. 385, 243–286 (1987). 38. Unwin, P. N. T. Nature 323, 12–13 (1986). 39. Deisenhofer, J., Epp, O., Miki, P., Huber, R. & Michel, H. Nature 318, 618–624 (1985). 40. Rodriguez, H. J. Chromatog. 350, 217–225 (1985). 41. Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981). 42. Hopp, T. P. & Woods, K. R. Proc. natn. Acad. Sci. U.S.A. 78, 3824–3828 (1981). 43. Staden, R. Nucleic Acids Res. 10, 2951–2961 (1982). 44. Barnard, E. A. & Bilbe, G. in Neurochemistry: A Practical Approach (eds Turner, A. J. & Bachelard, H.) 243–270 (IRL, Oxford, 1987). 45. Van Renterghem, C. et al. Molec. Brain Res. 2, 21–31 (1987).

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Schofield, P., Darlison, M., Fujita, N. et al. Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature 328, 221–227 (1987). https://doi.org/10.1038/328221a0

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