Abstract
In vitro mutagenesis of sequences required for the self-catalysed cleavage of a plant virus satellite RNA has allowed definition of an RNA segment with endoribonuclease activity. General rules have been deduced for the design of new RNA enzymes capable of highly specific RNA cleavage, and have been successfully tested against a new target sequence.
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Haseloff, J., Gerlach, W. Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature 334, 585–591 (1988). https://doi.org/10.1038/334585a0
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DOI: https://doi.org/10.1038/334585a0
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