Abstract
NATURAL killer (NK) cells are a subset of lymphocytes that mediate major histocompatibility complex (MHC)-nonrestricted cytotoxicity against tumours and virus-infected cells and secrete numerous cytokines on activation1. NK cells are distinct from mature T lymphocytes, because they do not rearrange or productively transcribe T-cell receptor α-, β-, γ- or δ-chain genes and do not express the CD3 γ- or δ-subunits2–6. But recent studies indicate that NK cells do express CD3ζ, co-associated with other membrane proteins7. Here we report that CD16, the receptor for the Fc (constant) region of IgG, specifically associates with the CD3ζ homodimer on the membrane of human NK cells, and that co-transfection with CD3ζ complementary DNA permits expression of a transmembrane-linked CD 16 complex on COS-7 cells. These findings indicate that CD3ζ can co-associate with membrane receptors of diverse ceil types and function as a common structure for signal transduction.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lanier, L. L., Phillips, J. H., Hackett, J. Jr., Tutt, M. & Kumar, V. J. Immun. 137, 2735–2739 (1986).
Ritz, J. et al. Science 228, 1540–1543 (1985).
Lanier, L. L., Cwirla, S. & Phillips, J. H. J Immun. 137, 3375–3377 (1986).
Lanier, L. L., Cwirla, S., Federspiel, N. & Phillips, J. H. J. exp. Med. 163, 209–214 (1986).
Loh, E. Y., Cwirla, S., Serafini, A. T., Phillips, J. H. & Lanier, L. L. Proc. natn. Acad Sci. U.S.A. 85, 9714–9718 (1988).
Biassoni, R., Ferrini, S., Prigione, I., Moretta, A. & Long, E. O. J. Immun. 140, 1685–1689 (1988).
Anderson, P., Caligiuri, M., Ritz, J. & Schlossman, S. F. Nature 341, 159–162 (1989).
Weissman, A. N. et al. Proc. natn. Acad. Sci. U.S.A. 85, 9709–9713 (1988).
Simmons, D. & Seed, B. Nature 333, 568–570 (1988).
Selvaraj, P., Rosse, W. F., Silber, R. & Springer, T. A. Nature 333, 565–567 (1988).
Huizinga, T. W. J. et al. Nature 333, 667–669 (1988).
Cassatella, M. A. et al. J. exp. Med. 169, 549–567 (1989).
Lanier, L. L., Ruitenberg, J. J. & Phillips, J. H. J. Immun. 141, 3478–3485 (1988).
Samelson, L. E. et al. J. Immun. 139, 2708–2714 (1987).
Samelson, L. E., Patel, M. D., Weissman, A. M., Harford, J. B. & Klausner, R. D. Cell 46, 1083–1090 (1989).
Mercep, M. et al. Science 242, 571–574 (1988).
Sussman, J. J. et al. Cell 52, 85–95 (1988).
Lanier, L. L., Le, A. M., Civin, C. I., Loken, M. R. & Phillips, J. H. J. Immun. 136, 4480–4486 (1986).
Nagler, A., Lanier, L. L., Cwirla, S. & Phillips, J. H. J. Immun. 143, 3183–3191 (1989).
Ravetch, J. V. & Perussia, B. J. exp. Med. 170, 481–497 (1989).
Lanier, L. L., Phillips, J. H. & Testi, R. Eur. J. Immun. 19, 775–778 (1989).
Ravetch, J. Int Congr. Immun (1989).
Miller, L., Blank, U., Metzger, H. & Kinet, J.-P. Science 244, 334–337 (1989).
Blank, U., Ra, C., White, K., Metzger, H. & Kinet, J.-P. Nature 337, 187–189 (1989).
Lanier, L. L. et al. J. exp. Med. 165, 1076–1094 (1987).
Weissman, A. M., Samelson, L. E. & Klausner, R. D. Nature 324, 480–482 (1986).
Cleveland, D. W., Fischer, S. G., Kirschner, M. W. & Laemmli, U. K. J. biol Chem. 252, 1102–1106 (1977).
Lanier, L. L., Cwirla, S., Yu, G., Testi, R. & Phillips, J. H. Science (in the press).
Seed, B. & Aruffo, A. Proc. natn. Acad. Sci. U.S.A. 84, 3365–3369 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lanier, L., Yu, G. & Phillips, J. Co-association of CD3ζ with a receptor (CD16) for IgG Fc on human natural killer cells. Nature 342, 803–805 (1989). https://doi.org/10.1038/342803a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/342803a0
This article is cited by
-
Leveraging CD16 fusion receptors to remodel the immune response for enhancing anti-tumor immunotherapy in iPSC-derived NK cells
Journal of Hematology & Oncology (2023)
-
The role of natural killer cell in gastrointestinal cancer: killer or helper
Oncogene (2021)
-
ImmuSort, a database on gene plasticity and electronic sorting for immune cells
Scientific Reports (2015)
-
NK and NKT Cells in Aging and Longevity: Role of Zinc and Metallothioneins
Journal of Clinical Immunology (2009)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.