Abstract
Voltage-dependent potassium, sodium and calcium ion channels may share a common mechanism of activation, in which the conserved S4 sequence acts as the primary voltage sensor. Site-directed mutagenesis of the S4 sequence of the Shaker potassium channel and electrophysiological analysis suggest that voltage-dependent activation involves the S4 sequence but is not solely due to electrostatic interactions.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hitle, B. Ionic Channels of Excitable Membranes (Sinauer, Sunderland, Massachusetts, 1984).
Zagotta, W. N. & Aldrich, R. W. J. gen. Physiol. 95, 29–60 (1990).
Armstrong, C. M. & Bezanilla, F. J. gen. Physiol. 63, 533–552 (1974).
Bezanilla, F., White, M. M. & Taylor, R. E. Nature 296, 657–659 (1982).
Tempel, B. L., Papazian, D. M., Schwarz, T. L., Jan, Y. N. & Jan, L. Y. Science 237, 770–775 (1987).
Noda, M. et al. Nature 320, 188–192 (1986).
Tanabe, T. et al. Nature 328, 313–318 (1987).
Tanabe, T., Beam, K. G., Powell, J. A. & Numa, S. Nature 336, 134–139 (1988).
Kosower, E. M. FEBS Lett. 182, 234–242 (1985).
Greenblatt, R. E., Blatt, Y. & Montal, M. FEBS Lett. 193, 125–134 (1985).
Guy, H. R. & Seetharamulu, P. Proc. natn. Acad. Sci. U.S.A. 83, 508–512 (1986).
Catterall, W. A. A. Rev. Biochem. 55, 953–985 (1986).
Stuhmer, W. et al. Nature 339, 597–603 (1989).
Armstrong, C. M. Physiol. Rev. 61, 644–683 (1981).
Papazian, D. M., Schwarz, T. L., Tempel, B. L., Jan, Y. N. & Jan, L. Y. Science 237, 749–753 (1987).
Kamb, A., Iverson, L. E. & Tanouye, M. A. Cell 50, 405–413 (1987).
Baumann, A. et al. EMBO J. 6, 3419–3429 (1987).
Schwarz, T. L., Tempel, B. L., Papazian, D. M., Jan, Y. N. & Jan, L. Y. Nature 331, 137–142 (1988).
Kamb, A., Tseng-Crank, J. & Tanouye, M. A. Neuron 1, 421–430 (1988).
Pongs, O. et al. EMBO J. 7, 1087–1096 (1988).
Timpe, L. C. et al. Nature 331, 143–145 (1988).
Timpe, L. C., Jan, Y. N. & Jan, L. Y. Neuron 1, 659–667 (1988).
Iverson, L. E., Tanouye, M. A., Lester, H. A., Davidson, N. & Rudy, B. Proc. natn. Acad. Sci. U.S.A. 85, 5723–5727 (1988).
Isacoff, E., Jan, Y. N. & Jan, L. Y. Nature 345, 530–534 (1990).
Ruppersberg, J. P. et al. Nature 345, 535–537 (1990).
Christie, M. J., North, R. A., Osborne, P. B., Douglass, J. & Adelman, J. P. Neuron 4, 405–411 (1990).
Almers, W. Rev. Physiol. Biochem. Pharmac. 82, 96–190 (1978).
Zagotta, W. N., Hoshi, T. & Aldrich, R. W. Proc. natn. Acad. Sci. U.S.A. 86, 7243–7247 (1989).
Armstrong, C. M., Bezanilla, F. & Rojas, E. J. gen. Physiol. 62, 375–391 (1973).
Goldman, L. Q. Rev. Biophys. 9, 491–526 (1976).
Armstrong, C. M. & Bezanilla, F. J. gen. Physiol. 70, 567–590 (1977).
Aldrich, R. W., Corey, D. P. & Stevens, C. F. Nature 306, 436–441 (1983).
Gonoi, T. & Hille, B. J. gen. Physiol. 89, 253–274 (1987).
Cota, G. & Armstrong, C. M. J. gen. Physiol. 94, 213–232 (1989).
Wells, J. A., Vasser, M. & Powers, D. B. Gene 34, 315–323 (1985).
Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor Laboratory, New York, 1989).
Kunkel, T. A., Roberts, J. D. & Zakour, R. A. Meth. Enzym. 154, 367–382 (1987).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Biggin, M. D., Gibson, T. J. & Hong, G. F. Proc. natn. Acad. Sci. U.S.A. 80, 3963–3965 (1983).
Zagotta, W. N., Brainard, M. S. & Aldrich, R. W. J. Neurosci. 8, 4765–4779 (1988).
Peres, A., Bernardini, G., Mancinelli, E. & Ferroni, A. Pflugers Arch. 403, 41–46 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Papazian, D., Timpe, L., Jan, Y. et al. Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence. Nature 349, 305–310 (1991). https://doi.org/10.1038/349305a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/349305a0
This article is cited by
-
Establishment and characterization of ZJUCHi003: an induced pluripotent stem cell line from a patient with Temple–Baraitser/Zimmermann–Laband syndrome carrying KCNH1 c.1070G > A (p.R357Q) variant
Human Cell (2024)
-
Ion currents through the voltage sensor domain of distinct families of proteins
Journal of Biological Physics (2023)
-
A constricted opening in Kir channels does not impede potassium conduction
Nature Communications (2020)
-
The HCN channel voltage sensor undergoes a large downward motion during hyperpolarization
Nature Structural & Molecular Biology (2019)
-
Independent movement of the voltage sensors in KV2.1/KV6.4 heterotetramers
Scientific Reports (2017)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.