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Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptor

Abstract

ANGIOTENSIN II elicits different responses which affect cardiovascular, neuronal and electrolyte transport regulation1. To under-stand the mechanisms responsible for its various actions, the receptor for angiotensin II has long been sought, but numerous attempts to purify the receptor have been unsuccessful owing to its instability and low concentration2–6. We report here the expression cloning of a complementary DNA encoding a bovine angiotensin II receptor to overcome these difficulties. The receptor cDNA encodes a protein of 359 amino-acid residues with a trans-membrane topology similar to that of other G protein-coupled receptors. COS-7 cells transfected with the cDNA expressed specific and high-affinity binding sites for angiotensin II, angiotensin II antagonist and a non-peptide specific antagonist for type-1 receptor. Dithiothreitol inhibited ligand binding. The con-centration of intracellular Ca2+ and of inositol-l,4,5-trisphosphate increased in the transfected COS-7 cells in response to angiotensin II or angiotensin III, indicating that this receptor is the type-1 receptor for angiotensin II. Northern blot analysis revealed that the messenger RNA for this receptor is expressed in bovine adrenal medulla, cortex and kidney.

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References

  1. Peach, M. J. Physiol. Rev. 57, 313–370 (1977).

    Article  CAS  Google Scholar 

  2. Capponi, A. M. & Catt, K. J. J. biol. Chem. 255, 12081–12086 (1980).

    CAS  PubMed  Google Scholar 

  3. Guillemetts, G. & Esher, E. Biochemistry 22, 5591–5596 (1983).

    Article  Google Scholar 

  4. Paglin, S. & Jamieson, J. D. Proc. natn. Acad. Sci. U.S.A. 79, 3739–3743 (1982).

    Article  ADS  CAS  Google Scholar 

  5. Rogers, T. B. J. biol. Chem. 259, 8106–8144 (1984).

    CAS  PubMed  Google Scholar 

  6. Akiyama, F., Imai, N., Hirose, S. & Murakami, K. Biomed. Res. 5, 9–18 (1984).

    Article  CAS  Google Scholar 

  7. Seed, B. Nature 329, 840–842 (1987).

    Article  ADS  CAS  Google Scholar 

  8. Carson, M. C., Leach-Harper, C. M., Baukal, A. J., Aguilera, G. & Catt, K. J. Molec. Endocr. 1, 147–153 (1987).

    Article  CAS  Google Scholar 

  9. Rondeau, J-J, et al. Biochem J. 268, 443–448 (1990).

    Article  CAS  Google Scholar 

  10. Jackson, T. R., Blair, L. A. C., Marshall, J., Goedert, M. & Hanley, M. R. Nature 355, 437–440 (1988).

    Article  ADS  Google Scholar 

  11. Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981).

    Article  CAS  Google Scholar 

  12. Kemp, B. E. & Pearson, R. B. Trends Biochem. Sci. 15, 342–346 (1990).

    Article  CAS  Google Scholar 

  13. Strader, C. D., Irving, S. S. & Richard, A. F. FASEB J. 3, 1825–1832 (1989).

    Article  CAS  Google Scholar 

  14. O'Dowd, B., Hnatowich, M., Caron, M. G., Lefkowitz, R. J. & Bouvier, M. J. biol. Chem. 264, 7564–7569 (1989).

    CAS  PubMed  Google Scholar 

  15. Ovchinnikov, Y., Abdulaev, N. & Bogachuk, A. FEBS Lett. 230, 1–5 (1988).

    Article  CAS  Google Scholar 

  16. Chang, R. S. L., Lotti, V. J. & Keegan, M. Biochem. Pharmac. 31, 1903–1906 (1982).

    Article  CAS  Google Scholar 

  17. Peach, M. J. & Dostal, D. E. J. Cardiovasc. Pharmac. 16, S25–S30 (1990).

    Article  CAS  Google Scholar 

  18. Glossmann, H., Baukal, J. & Catt, K. J. J. J. biol. Chem. 294, 825–834 (1974).

    Google Scholar 

  19. Dudley, D. T. et al. Molec. Pharmac. 38, 370–377 (1990).

    CAS  Google Scholar 

  20. Skorecki, K. L., Ballerman, B. J., Rennke, H. G. & Brenner, B. M. Fed. Proc. 42, 3064–3070 (1983).

    CAS  PubMed  Google Scholar 

  21. Chiu, A. T. et al. Biochem. biophys. Res. Commun. 165, 196–203 (1989).

    Article  CAS  Google Scholar 

  22. Whitebread, S., Michéle, M., Kamber, B. & de Gaspero, M. Biochem. biophys. Res. Commun. 163, 284–291 (1989).

    Article  CAS  Google Scholar 

  23. Birnstiel, M. L., Busslinger, M. & Strub, K. Cell 41, 349–359 (1985).

    Article  CAS  Google Scholar 

  24. Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).

    Article  CAS  Google Scholar 

  25. Hirt, B. J. molec. Biol. 26, 365–369 (1967).

    Article  CAS  Google Scholar 

  26. Zhou, Q-Y. et al. Nature 347, 76–80 (1990).

    Article  ADS  CAS  Google Scholar 

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Sasaki, K., Yamano, Y., Bardhan, S. et al. Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptor. Nature 351, 230–233 (1991). https://doi.org/10.1038/351230a0

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