Abstract
ANGIOTENSIN II elicits different responses which affect cardiovascular, neuronal and electrolyte transport regulation1. To under-stand the mechanisms responsible for its various actions, the receptor for angiotensin II has long been sought, but numerous attempts to purify the receptor have been unsuccessful owing to its instability and low concentration2–6. We report here the expression cloning of a complementary DNA encoding a bovine angiotensin II receptor to overcome these difficulties. The receptor cDNA encodes a protein of 359 amino-acid residues with a trans-membrane topology similar to that of other G protein-coupled receptors. COS-7 cells transfected with the cDNA expressed specific and high-affinity binding sites for angiotensin II, angiotensin II antagonist and a non-peptide specific antagonist for type-1 receptor. Dithiothreitol inhibited ligand binding. The con-centration of intracellular Ca2+ and of inositol-l,4,5-trisphosphate increased in the transfected COS-7 cells in response to angiotensin II or angiotensin III, indicating that this receptor is the type-1 receptor for angiotensin II. Northern blot analysis revealed that the messenger RNA for this receptor is expressed in bovine adrenal medulla, cortex and kidney.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Peach, M. J. Physiol. Rev. 57, 313–370 (1977).
Capponi, A. M. & Catt, K. J. J. biol. Chem. 255, 12081–12086 (1980).
Guillemetts, G. & Esher, E. Biochemistry 22, 5591–5596 (1983).
Paglin, S. & Jamieson, J. D. Proc. natn. Acad. Sci. U.S.A. 79, 3739–3743 (1982).
Rogers, T. B. J. biol. Chem. 259, 8106–8144 (1984).
Akiyama, F., Imai, N., Hirose, S. & Murakami, K. Biomed. Res. 5, 9–18 (1984).
Seed, B. Nature 329, 840–842 (1987).
Carson, M. C., Leach-Harper, C. M., Baukal, A. J., Aguilera, G. & Catt, K. J. Molec. Endocr. 1, 147–153 (1987).
Rondeau, J-J, et al. Biochem J. 268, 443–448 (1990).
Jackson, T. R., Blair, L. A. C., Marshall, J., Goedert, M. & Hanley, M. R. Nature 355, 437–440 (1988).
Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555–583 (1981).
Kemp, B. E. & Pearson, R. B. Trends Biochem. Sci. 15, 342–346 (1990).
Strader, C. D., Irving, S. S. & Richard, A. F. FASEB J. 3, 1825–1832 (1989).
O'Dowd, B., Hnatowich, M., Caron, M. G., Lefkowitz, R. J. & Bouvier, M. J. biol. Chem. 264, 7564–7569 (1989).
Ovchinnikov, Y., Abdulaev, N. & Bogachuk, A. FEBS Lett. 230, 1–5 (1988).
Chang, R. S. L., Lotti, V. J. & Keegan, M. Biochem. Pharmac. 31, 1903–1906 (1982).
Peach, M. J. & Dostal, D. E. J. Cardiovasc. Pharmac. 16, S25–S30 (1990).
Glossmann, H., Baukal, J. & Catt, K. J. J. J. biol. Chem. 294, 825–834 (1974).
Dudley, D. T. et al. Molec. Pharmac. 38, 370–377 (1990).
Skorecki, K. L., Ballerman, B. J., Rennke, H. G. & Brenner, B. M. Fed. Proc. 42, 3064–3070 (1983).
Chiu, A. T. et al. Biochem. biophys. Res. Commun. 165, 196–203 (1989).
Whitebread, S., Michéle, M., Kamber, B. & de Gaspero, M. Biochem. biophys. Res. Commun. 163, 284–291 (1989).
Birnstiel, M. L., Busslinger, M. & Strub, K. Cell 41, 349–359 (1985).
Shaw, G. & Kamen, R. Cell 46, 659–667 (1986).
Hirt, B. J. molec. Biol. 26, 365–369 (1967).
Zhou, Q-Y. et al. Nature 347, 76–80 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sasaki, K., Yamano, Y., Bardhan, S. et al. Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptor. Nature 351, 230–233 (1991). https://doi.org/10.1038/351230a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/351230a0
This article is cited by
-
Obituary: Dr. Tadashi Inagami, a legend in the field of renin angiotensin II research
Hypertension Research (2023)
-
The Renin Angiotensin System as a Therapeutic Target in Traumatic Brain Injury
Neurotherapeutics (2023)
-
A historical perspective on ACE2 in the COVID-19 era
Journal of Human Hypertension (2021)
-
Probing the structural requirements for angiotensin II receptor: molecular modeling studies
Network Modeling Analysis in Health Informatics and Bioinformatics (2018)
-
Discovery of Potent Antihypertensive Ligands Substituted Imidazolyl Biphenyl Sulfonylureas Analogs as Angiotensin II \(\hbox {AT}_{1}\) AT 1 Receptor Antagonists by Molecular Modelling Studies
Interdisciplinary Sciences: Computational Life Sciences (2015)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.