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Phosducin is a protein kinase A-regulated G-protein regulator

Nature volume 358, pages 73–76 (1992)Cite this article

Abstract

SIGNAL transduction by G-protein-coupled receptors is regulated by various mechanisms acting at the receptor level; those studied most thoroughly are from the β-adrenergic receptor/Gs/adenylyl cyclase system1–3. We report here a regulatory mechanism occurring at the level of the G proteins themselves. A protein with Mr 33,000 that inhibits Gs-GTPase activity was purified from bovine brain. This protein is very similar or identical to phosducin, a protein previously thought to be specific for retina and pineal gland4–8. Recombinant phosducin inhibited the GTPase activity of several G proteins, and also inhibited Gs-mediated adenylyl cyclase activation. Blockade of its inhibitory effects by protein kinase A suggests that phosducin may be part of a complex regulatory network controlling G-protein-mediated signalling.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, University of Munich, Max-Planck-Institute of Biochemistry, 8033, Martinsried, Germany

    Petra H. Bauer, Stefan Müller, Mechthild Puzicha, Susanne Pippig, Brigitte Obermaier & Martin J. Lohse

  2. Department of Physiological Chemistry, University of Wurzburg, Medical School, 8700, Wurzburg, Germany

    Stefan Müller & Ernst J. M. Helmreich

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  1. Petra H. Bauer
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Bauer, P., Müller, S., Puzicha, M. et al. Phosducin is a protein kinase A-regulated G-protein regulator. Nature 358, 73–76 (1992). https://doi.org/10.1038/358073a0

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