Abstract
PARASYMPATHETIC nerve stimulation causes slowing of the heart rate by activation of muscarinic receptors and the subsequent opening of muscarinic K+ channels in the sinoatrial node and atrium1–4. This inwardly rectifying K+ channel is coupled directly with G protein5–10. Based on sequence homology with cloned inwardly rectifying K+ channels, ROMK1 (ref. 11) and IRK1 (ref. 12), we have isolated a complementary DNA for a G-protein-coupled inwardly rectifying K+ channel (GIRK1) from rat heart. The GIRK1 channel probably corresponds to the muscarinic K channel because (1) its functional properties resemble those of the a trial muscarinic K+ channel and (2) its messenger RNA is much more abundant in the atrium than in the ventricle. In addition, GIRK1 mRNA is expressed not only in the heart but also in the brain.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Trautwein, W. & Dudel, J. Pflügers Arch. 266, 324–334 (1958).
Noma, A., Peper, K. & Trautwein, W. Pflügers Arch. 381, 255–262 (1979).
Sakmann, B., Noma, A. & Trautwein, W. Nature 303, 250–253 (1983).
Soejima, M. & Noma, A. Pflügers Arch. 400, 424–431 (1984).
Breitwieser, G. E. & Szabo, G. Nature 317, 538–540 (1985).
Logothetis, D. E., Kurachi, Y., Galper, J., Neer, E. J. & Clapham, D. E. Nature 325, 321–326 (1987).
Yatani, A., Codina, J., Brown, A. M. & Birnbaumer, L. Science 235, 207–211 (1987).
Yatani, A. et al. Nature 336, 680–682 (1988).
Brown, A. M. & Birnbaumer, L. A. Rev. Physiol. 52, 197–213 (1990).
Kurachi, Y., Tung, R. T., Ito, H. & Nakajima, T. Prog. Neurobiol. 39, 229–246 (1992).
Ho, K. et al. Nature 362, 31–38 (1993).
Kubo, Y., Baldwin, T. J., Jan, Y. N. & Jan, L. Y. Nature 362, 127–133 (1993).
Bonner, T. I., Buckley, N. J., Young, A. C. & Brann, M. R. Science 237, 527–532 (1987).
Gilman, A. G. Cell 36, 577–579 (1984).
Dascal, N. Crit. Rev. Biochem. 22, 317–387 (1987).
Jones, D. T. & Reed, R. R. J. biol. Chem. 262, 14241–14249 (1987).
Fong, H. K. W. et al. Proc. natn. Acad. Sci. U.S.A. 83, 2162–2166 (1986).
Gautam, N., Baetscher, M., Aebersold, R. & Simon, M. I. Science 244, 971–974 (1989).
Ito, H. et al. J. gen. Physiol. 98, 517–533 (1991).
Morie, M. & Irisawa, H. J. Physiol. 408, 313–332 (1989).
Ashcroft, S. J. H. & Achcroft, F. M. Cell. Signal. 2, 197–214 (1990).
Vandongen, A. M. J. et al. Science 242, 1433–1437 (1988).
Brown, D. A. Rev. Physiol. 52, 215–242 (1990).
Stanfield, P. R., Nakajima, Y. & Yamaguchi, K. Nature 315, 498–501 (1985).
Gahwiler, B. H. & Brown, D. A. Proc. natn. Acad. Sci. U.S.A. 82, 1558–1562 (1985).
Williams, J. T., Colmers, W. F. & Pan, Z. Z. J. Neurosci. 8, 3499–3506 (1988).
Mihara, S., North, R. A. & Surprenant, A. J. Physiol. 390, 335–355 (1987).
Inoue, M., Nakajima, S. & Nakajima, Y. J. Physiol. 407, 177–198 (1988).
North, R. A., Williams, J. T., Surprenant, A. & Christie, M. J. Proc. natn. Acad. Sci. U.S.A. 84, 5487–5491 (1987).
Wimpey, T. L. & Chavkin, C. Neuron 6, 281–289 (1991).
Gerber, U., Stevens, D. R., McCarley, R. W. & Greene, R. W. J. Neurosci. 11, 3861–3867 (1991).
Frohman, M. A. PCR Protocols 28–38 (Academic, San Diego, 1990).
Baldwin, T. J. et al. Neuron 7, 471–483 (1991).
Ito, H. et al. J. gen. Physiol. 99, 961–983 (1992).
Yang, X.-C. & Sachs, F. Science 243, 1068–1071 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubo, Y., Reuveny, E., Slesinger, P. et al. Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel. Nature 364, 802–806 (1993). https://doi.org/10.1038/364802a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/364802a0
This article is cited by
-
Abnormal Electroretinogram after Kir7.1 Channel Suppression Suggests Role in Retinal Electrophysiology
Scientific Reports (2017)
-
Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model
Molecular and Cellular Biochemistry (2017)
-
G Protein-Gated Inwardly Rectifying Potassium Channel Subunits 1 and 2 are Down-Regulated in Rat Dorsal Root Ganglion Neurons and Spinal Cord after Peripheral Axotomy
Molecular Pain (2015)
-
The bundle crossing region is responsible for the inwardly rectifying internal spermine block of the Kir2.1 channel
Pflügers Archiv - European Journal of Physiology (2014)
-
X-ray structure of the mammalian GIRK2–βγ G-protein complex
Nature (2013)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.