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Protein disaggregation mediated by heat-shock protein Hspl04

Nature volume 372, pages 475–478 (1994)Cite this article

Abstract

THE heat-inducible members of the HsplOO (or Clp) family of proteins share a common function in helping organisms to survive extreme stress, but the basic mechanism through which these pro-teins function is not understood1–5. Hspl04 protects cells against a variety of stresses, under many physiological conditions6,7, and its function has been evolutionarily conserved, at least from Saccharomyces cerevisiae to Arabidopsis thaliana25. Homology with the Escherichia coli Clp A protein suggests that Hspl04 may provide stress tolerance by helping to rid the cell of heat-denatured proteins through proteolysis1. But genetic analysis indicates that Hspl04 may function like Hsp70 as a molecular chaperone8. Here we investigate the role of Hspl04 in vivo using a temperature-sensitiveVibrio harveyi luciferase-fusion protein as a test substrate9. We find that Hspl04 does not protect luciferase from thermal denaturation, nor does it promote proteolysis of luciferase. Rather, Hspl04 functions in a manner not previously described for other heat-shock proteins: it mediates the resolubilization of heat-inactivated luciferase from insoluble aggregates.

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Author notes

  1. Dawn A. Parsell

    Present address: Connective Therapeutics Inc., 3400 West Bayshore Road, Palo Alto, California, 94303, USA

Authors and Affiliations

  1. Department of Molecular Genetics and Cell Biology, The University of Chicago, MC1028 N339, 5841 S. Maryland Avenue, Chicago, Illinois, 60637, USA

    Dawn A. Parsell & Susan Lindquist

  2. Department of Pathology, The University of Chicago, MC1028 N339, 5841 S. Maryland Avenue, Chicago, Illinois, 60637, USA

    Mike A. Singer

  3. The Howard Hughes Medical Institute, The University of Chicago, MC1028 N339, 5841 S. Maryland Avenue, Chicago, Illinois, 60637, USA

    Dawn A. Parsell, Anthony S. Kowal & Susan Lindquist

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  2. Anthony S. Kowal
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  3. Mike A. Singer
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  4. Susan Lindquist
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Parsell, D., Kowal, A., Singer, M. et al. Protein disaggregation mediated by heat-shock protein Hspl04. Nature 372, 475–478 (1994). https://doi.org/10.1038/372475a0

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