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Defective antigen receptor-mediated proliferation of B and T cells in the absence of Vav

Nature volume 374, pages 467–470 (1995)Cite this article

Abstract

CROSSLINKING of B- or T-cell antigen receptors results in the rapid tyrosine phosphorylation of a number of proteins, including Vav, a protein expressed in cells of the haematopoietic system1–3. Vav contains an array of structural motifs that include Src-homology domains SH2/SH34 and regions of homology to the guanine-nucleotide-exchange protein Dbl, pleckstrin and protein kinase C (refs 5-9). Using the RAG-complementation approach10, we have analysed in vivo differentiation and in vitro responses of B-and T-lineage cells generated by injection of embryonic stem cells homozygous for a null mutation in the vav gene into blastocysts of RAG-1- or RAG-2-deficient mice. Here we report that antigen receptor-mediated proliferative responses of B and T cells in vitro are severely reduced in the absence of Vav. We also suggest a direct link between the low proliferative response of Vav-deficient B and T cells and the reduced number of these cells in peripheral lymphoid organs of chimaeric mice.

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Authors and Affiliations

  1. Institute for Genetics, University of Cologne, Weyertal 121, 50931, Cologne, Germany

    Alexander Tarakhovsky, Stefan Schaal & Klaus Rajewsky

  2. National Institute for Medical Research, The Ridgeway, London, NW7 1AA, UK

    Martin Turner, P. Joseph Mee, Linda P. Duddy & Victor L. J. Tybulewicz

Authors
  1. Alexander Tarakhovsky
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  2. Martin Turner
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  3. Stefan Schaal
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  7. Victor L. J. Tybulewicz
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Tarakhovsky, A., Turner, M., Schaal, S. et al. Defective antigen receptor-mediated proliferation of B and T cells in the absence of Vav. Nature 374, 467–470 (1995). https://doi.org/10.1038/374467a0

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