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The 2.0 Å crystal structure of a heterotrimeric G protein

Nature volume 379, pages 311–319 (1996)Cite this article

Abstract

The structure of a heterotrimeric G protein reveals the mechanism of the nucleotide-dependent engagement of the α and βγ subunits that regulates their interaction with receptor and effector molecules. The interaction involves two distinct interfaces and dramatically alters the conformation of the αbut not of theβγ subunits. The location of the known sites for posttranslational modification and receptor coupling suggest a plausible orientation with respect to the membrane surface and an activated heptahelical receptor.

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Author information

Author notes

  1. David G. Lambright

    Present address: Program in Molecular Medicine, University of Massachusetts Medical Center, 373 Plantation Street, Worcester, Massachusetts, 01605, USA

  2. Paul B. Sigler: To whom correspondence should be addressed.

Authors and Affiliations

  1. The Department of Molecular Biophysics and Biochemistry, and the Howard Hughes Medical Institute, Yale University, 295 Congress Avenue, BCMM 154, New Haven, Connecticut, 06510, USA

    David G. Lambright, John Sondek, Andrew Bohm & Paul B. Sigler

  2. Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, Illinois, 60680, USA

    Nikolai P. Skiba & Heidi E. Hamm

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Lambright, D., Sondek, J., Bohm, A. et al. The 2.0 Å crystal structure of a heterotrimeric G protein. Nature 379, 311–319 (1996). https://doi.org/10.1038/379311a0

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